| Detailed Information for Phage Chubster |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Sakina Hasan |
| Year Found | 2015 |
| Location Found | Lansdowne, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.940708 N, 75.261294 W Map |
| Discovery Notes | Found in my backyard in my uncle's garden, soil was near plants. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 15, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1361 |
| Genome length (bp) | 70258 |
| Character of genome ends | Circularly Permuted |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | plaques are small, round and clear
phage grows with various calcium chloride concentrations.Grows optimal with 5mM, 25mM and 50mM calcium chloride at room temperature and 30 degrees Celsius. Phage does not form plaques without calcium chloride or at 37 degrees Celsius. Plaques grow best with higher calcium chloride concentrations. Plaques also form with 5mM and 25mM Magnesium sulfate concentrations but, not as well as calcium chloride. |
| Morphotype | Myoviridae |
| Number of Genes | 112 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX670786 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
Cabrini College |
| SEA Lysate Titer |
3.1x10^7 |
| Date of SEA Lysate Titering |
Jun 30, 2015 |
| Pitt Freezer Box# |
149 |
| Pitt Freezer Box Grid# |
C1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
