Gordonia phage Dardanus
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| Detailed Information for Phage Dardanus | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Samantha J. Wills |
| Year Found | 2016 |
| Location Found | Fort Myers, FL USA |
| Finding Institution | Florida Gulf Coast University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 26.460278 N, 81.78 W Map |
| Discovery Notes | My bacteriophage was found in the Food Forest at Florida Gulf Coast University. The soil was dry and sandy. My bacteriophage was both enriched and plated on the bacteria Gordonia terrae. |
| Naming Notes | My bacteriophage is named after the Greek Demigod Dardanus. Dardanus is the son of Zeus and Electra, and the grandson of Atlas according to Greek mythology. This name is meant to reflect my bacteriophage's power. I view my bacteriophage as powerful because it covered my entire plate at the beginning of a serial dilution series. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 278 |
| Genome length (bp) | 43143 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCGGCAGGTA |
| GC Content | 66.6% |
| Sequencing Notes | The sequencing reads from this phage showed that there were at least two deletions in a subset of the population, including one that was present in more than half of the population. The final fasta file that is posted contains these deleted regions, even though the first one is present in a minority of the actual phage particles. Deletion #1 is found in about 70% of the reads, and is a 1218 bp deletion from 25156 to 26373, inclusive. Deletion #2 is found in about 20% of the reads that do NOT have deletion #1, and is 901 bp from 25320 to 26220, inclusive. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CU |
| Subcluster | CU3 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | My plaques were round and clear. |
| Other Cluster Members | |
| Annotating Institution | University of Louisiana at Monroe |
| Annotation Status | In GenBank |
| Plaque Notes | My phage came from a lyctic plaque inside a turbid plaque. When plated on gordonia terrae for a serial dillution series, small clear plaques developed, as well as large turbid plaques. The least amount of plaques found on a plate was 10 plaques. My plaques are large, however they vary in size. My plaques have a diameter of approximately 2-3.5 millimeters. |
| Number of Genes | 72 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN010758 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 44 |
| Pitt Freezer Box Grid# | G10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |