Detailed Information for Phage Daredevil |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Former names | Aragorn |
Found By | Elizabeth Crinzi |
Year Found | 2017 |
Location Found | Pittsburgh, PA USA |
Finding Institution | University of Pittsburgh |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 40.439444 N, 79.954167 W Map |
Discovery Notes | This phage was found in a soil sample collected at Posvar Hall in Pittsburgh, PA on January 8th, 2017 at 3:49 PM EST. The temperature at the time of collection was 16 degrees Fahrenheit, the humidity was 47%, and the weather was cloudy with a 12 mph wind. The day's high temperature 17 degrees Fahrenheit and the low was 8 degrees Fahrenheit. The soil sample was stored in a cool, dark drawer at room temperature for approximately 20 hours before being used in lab. The sample was then directly isolated and incubated for approximately 30 degrees Celsius for three days |
Naming Notes | I named this phage Daredevil after one of my favorite superheroes and tv shows. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | May 15, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 680 |
Genome length (bp) | 90490 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGCAACGCTC |
GC Content | 64.8% |
Sequencing Notes | at position 46745 bp approximately 28% of reads contain a C instead of consensus G |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | DL |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | University of Pittsburgh |
Annotation Status | In GenBank |
Plaque Notes | Plaques are about 1.7 mm in diameter. The plaques are clear. |
Morphotype | Siphoviridae |
Number of Genes | 165 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MH590603 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
55 |
Pitt Freezer Box Grid# |
E7 |
Available Files |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |