Mycobacterium phage Farewell
Farewell Plaque Picture
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Detailed Information for Phage Farewell
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDeborah Jacobs-Sera
Year Found2023
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.446336 N, 79.954178 W Map
Discovery NotesFound outside Langley Hall in a mulched bed.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 5, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1700
Genome length (bp)61741
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGCAT
GC Content65.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAF
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members

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Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque Notesplaques are ~5mm, with a clear center and turbid halos.
MorphotypeSiphoviridae
Number of Genes92
Number of tRNAs4
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Farewell_1
(terminase small subunit)

Farewell_2
(terminase large subunit)

Farewell_3
(portal protein)

Farewell_4
(capsid maturation protease)

Farewell_5
(scaffolding protein)

Farewell_6
(major capsid protein)

Farewell_7
(head-to-tail adaptor)

Farewell_8
(head-to-tail stopper)

Farewell_9

Farewell_10
(tail terminator)

Farewell_11
(major tail protein)

Farewell_12
(tail assembly chaperone)

Farewell_13
(tail assembly chaperone)

Farewell_14
(tape measure protein)

Farewell_15
(minor tail protein)

Farewell_16
(minor tail protein)

Farewell_17
(minor tail protein)

Farewell_18

Farewell_19
(minor tail protein)

Farewell_20

Farewell_21
(minor tail protein)

Farewell_22

Farewell_23

Farewell_24

Farewell_25
(lysin A)

Farewell_26

Farewell_27
(lysin B)

Farewell_28
(holin)

Farewell_29

Farewell_30

Farewell_31 — tRNA
(tRNA-Ser(gct))

Farewell_32 — tRNA
(tRNA-Pro(tgg))

Farewell_33 — tRNA
(tRNA-Trp(cca))

Farewell_34 — tRNA
(tRNA-Tyr(gta))

Farewell_35

Farewell_36
(Ro-like RNA binding protein)

Farewell_37
(helix-turn-helix DNA binding protein)

Farewell_38

Farewell_39

Farewell_40

Farewell_41

Farewell_42

Farewell_43

Farewell_44
(tyrosine integrase)

Farewell_45

Farewell_46

Farewell_47

Farewell_48
(immunity repressor)

Farewell_49
(helix-turn-helix DNA binding protein)

Farewell_50
(recombination directionality factor)

Farewell_51

Farewell_52

Farewell_53

Farewell_54

Farewell_55

Farewell_56

Farewell_57
(DnaQ-like DNA polymerase III subunit)

Farewell_58
(RecE-like exonuclease)

Farewell_59
(RecT-like DNA pairing protein)

Farewell_60

Farewell_61

Farewell_62

Farewell_63

Farewell_64

Farewell_65
(DNA binding protein)

Farewell_66
(DNA methylase)

Farewell_67

Farewell_68
(DNA methylase)

Farewell_69

Farewell_70

Farewell_71

Farewell_72

Farewell_73

Farewell_74
(methyltransferase)

Farewell_75

Farewell_76
(WhiB family transcription factor)

Farewell_77

Farewell_78
(DprA-like DNA processing chain A)

Farewell_79

Farewell_80

Farewell_81
(DnaQ-like DNA polymerase III subunit)

Farewell_82
(RepA-like replication initiator)

Farewell_83

Farewell_84

Farewell_85

Farewell_86

Farewell_87

Farewell_88
(excise protein)

Farewell_89
(glycosyltransferase)

Farewell_90
(glycosyltransferase)

Farewell_91
(RelB-like antitoxin)

Farewell_92
(RelE-like toxin)

Farewell_93

Farewell_94

Farewell_95

Farewell_96
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP750958
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
EM PictureDownload
GenBank File for PhameratorDownload