Mycobacterium phage Farewell
Farewell Plaque Picture
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Detailed Information for Phage Farewell
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDeborah Jacobs-Sera
Year Found2023
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.446336 N, 79.954178 W Map
Discovery NotesFound outside Langley Hall in a mulched bed.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 5, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina MiSeq
Read TypeSingle-end reads
Read Length150 bp
Approximate Shotgun Coverage1700
Genome length (bp)61741
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGCAT
GC Content65.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAF
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members

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Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque Notesplaques are ~5mm, with a clear center and turbid halos.
MorphotypeSiphoviridae
Number of Genes92
Number of tRNAs4
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Farewell_1
(terminase small subunit)

Farewell_2
(terminase large subunit)

Farewell_3
(portal protein)

Farewell_4
(capsid maturation protease)

Farewell_5
(scaffolding protein)

Farewell_6
(major capsid protein)

Farewell_7
(head-to-tail adaptor)

Farewell_8
(head-to-tail stopper)

Farewell_9

Farewell_10
(tail terminator)

Farewell_11
(major tail protein)

Farewell_12
(tail assembly chaperone)

Farewell_13
(tail assembly chaperone)

Farewell_14
(tape measure protein)

Farewell_15
(minor tail protein)

Farewell_16
(minor tail protein)

Farewell_17
(minor tail protein)

Farewell_18

Farewell_19
(minor tail protein)

Farewell_20

Farewell_21
(minor tail protein)

Farewell_22

Farewell_23

Farewell_24

Farewell_25
(lysin A)

Farewell_26

Farewell_27
(lysin B)

Farewell_28
(holin)

Farewell_29

Farewell_30

Farewell_31 — tRNA
(tRNA-Ser(gct))

Farewell_32 — tRNA
(tRNA-Pro(tgg))

Farewell_33 — tRNA
(tRNA-Trp(cca))

Farewell_34 — tRNA
(tRNA-Tyr(gta))

Farewell_35

Farewell_36
(Ro-like RNA binding protein)

Farewell_37
(helix-turn-helix DNA binding protein)

Farewell_38

Farewell_39

Farewell_40

Farewell_41

Farewell_42

Farewell_43

Farewell_44
(tyrosine integrase)

Farewell_45

Farewell_46

Farewell_47

Farewell_48
(immunity repressor)

Farewell_49
(helix-turn-helix DNA binding protein)

Farewell_50
(recombination directionality factor)

Farewell_51

Farewell_52

Farewell_53

Farewell_54

Farewell_55

Farewell_56

Farewell_57
(DnaQ-like DNA polymerase III subunit)

Farewell_58
(RecE-like exonuclease)

Farewell_59
(RecT-like DNA pairing protein)

Farewell_60

Farewell_61

Farewell_62

Farewell_63

Farewell_64

Farewell_65
(DNA binding protein)

Farewell_66
(DNA methylase)

Farewell_67

Farewell_68
(DNA methylase)

Farewell_69

Farewell_70

Farewell_71

Farewell_72

Farewell_73

Farewell_74
(methyltransferase)

Farewell_75

Farewell_76
(WhiB family transcription factor)

Farewell_77

Farewell_78
(DprA-like DNA processing chain A)

Farewell_79

Farewell_80

Farewell_81
(DnaQ-like DNA polymerase III subunit)

Farewell_82
(RepA-like replication initiator)

Farewell_83

Farewell_84

Farewell_85

Farewell_86

Farewell_87

Farewell_88
(excise protein)

Farewell_89
(glycosyltransferase)

Farewell_90
(glycosyltransferase)

Farewell_91
(RelB-like antitoxin)

Farewell_92
(RelE-like toxin)

Farewell_93

Farewell_94

Farewell_95

Farewell_96
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP750958
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 220
Pitt Freezer Box Grid# C8
Available Files
EM PictureDownload
GenBank File for PhameratorDownload