Mycobacterium phage FourStarNutty
	
	
	
	
	
	
	
	
        
	
	
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	| Detailed Information for Phage FourStarNutty | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 | 
| Found By | Allyson Roberts | 
| Year Found | 2012 | 
| Location Found | Baltimore, MD USA | 
| Finding Institution | Johns Hopkins University | 
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science | 
| From enriched soil sample? | Yes | 
| Isolation Temperature | Not entered | 
| GPS Coordinates | Unavailable | 
| Discovery Notes | The soil gathered was taken from a community garden of Johns Hopkins University. It was collected at approximately 10:30am, from approximately 4cm beneath the surface. The soil was fairly dry and was not directly next to or surrounding any live plants. | 
| Naming Notes | After coming into the lab outside of my class on a Friday afternoon, I developed a stronger bond with my professor. Both of us are fairly sassy, and we bantered back and forth. By the end of the day, my professor made a comment about how he was going to have a "Four-Star Nutty." Apparently it refers to some sort of fit, and was a popular phrase "back in the day." While Google doesn't return a single result defining the term as my professor had, and I'm not sure the phrase is legitimate at all, it seemed like a fitting name for my temperamental and aggressive phage (while also poking fun at my professor). | 
| Sequencing Information | |
| Sequencing Complete? | No | 
| Genome length (bp) | Unknown | 
| Character of genome ends | Unknown | 
| Fasta file available? | No | 
| Characterization | |
| Cluster | A | 
| Subcluster | A1 | 
| Cluster Life Cycle | Temperate | 
| Annotating Institution | Unknown or unassigned | 
| Annotation Status | Not sequenced | 
| Plaque Notes | The plaques are of a bulls-eye morphology, and typically appear to be approximately 3-5mm in diameter (however, if two or more plaques merge together, they can sometimes appear to be a single, larger plaque). The plaques are often fairly aggressive, typically either completely covering a plate with plaques or having no plaques on a plate. | 
| Morphotype | Siphoviridae | 
| Has been Phamerated? | No | 
| Publication Info | |
| Uploaded to GenBank? | No | 
| GenBank Accession | None yet | 
| Refseq Number | None yet | 
| Archiving Info | |
| Archiving status | Archived | 
| SEA Designator | FourStarNutty | 
| SEA Lysate Titer | 8.1 x 109 pfu/mL | 
| Date of SEA Lysate Titering | Oct 11, 2012 | 
| Pitt Freezer Box# | 13 | 
| Pitt Freezer Box Grid# | B1 | 
| Available Files | |
| Plaque Picture | Download | 
| Restriction Digest Picture | Download | 
| EM Picture | Download | 
 
		