Mycobacterium phage FourStarNutty
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Detailed Information for Phage FourStarNutty | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Allyson Roberts |
Year Found | 2012 |
Location Found | Baltimore, MD USA |
Finding Institution | Johns Hopkins University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | Unavailable |
Discovery Notes | The soil gathered was taken from a community garden of Johns Hopkins University. It was collected at approximately 10:30am, from approximately 4cm beneath the surface. The soil was fairly dry and was not directly next to or surrounding any live plants. |
Naming Notes | After coming into the lab outside of my class on a Friday afternoon, I developed a stronger bond with my professor. Both of us are fairly sassy, and we bantered back and forth. By the end of the day, my professor made a comment about how he was going to have a "Four-Star Nutty." Apparently it refers to some sort of fit, and was a popular phrase "back in the day." While Google doesn't return a single result defining the term as my professor had, and I'm not sure the phrase is legitimate at all, it seemed like a fitting name for my temperamental and aggressive phage (while also poking fun at my professor). |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | A |
Subcluster | A1 |
Cluster Life Cycle | Temperate |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | The plaques are of a bulls-eye morphology, and typically appear to be approximately 3-5mm in diameter (however, if two or more plaques merge together, they can sometimes appear to be a single, larger plaque). The plaques are often fairly aggressive, typically either completely covering a plate with plaques or having no plaques on a plate. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Designator | FourStarNutty |
SEA Lysate Titer | 8.1 x 109 pfu/mL |
Date of SEA Lysate Titering | Oct 11, 2012 |
Pitt Freezer Box# | 13 |
Pitt Freezer Box Grid# | B1 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |