Detailed Information for Phage Zetzy |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Tessa Retzlaff |
Year Found | 2013 |
Location Found | Clinton, NE USA |
Finding Institution | Chadron State College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 42.750243 N, 102.333481 W Map |
Discovery Notes | The soil sample was collected about an inch deep in the middle of an alfalfa field. After a nice rain the night before. The time was at 9:16 in the morning at 26C. More than one phage was in this soil sample. |
Naming Notes | The name for the phage is the nickname for my past twin brother, who always made fun of me for being a nerd. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 29, 2014 |
Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
Shotgun Sequencing Method | 454 Pyrosequencing |
Approximate Shotgun Coverage | 83 |
Sanger Finishing Reactions | 6 |
Genome length (bp) | 48229 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGGTGGTAA |
GC Content | 64.0% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A3 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | University of Pittsburgh |
Annotation Status | In GenBank |
Plaque Notes | after 48hrs diameter: 3mm both turbid and clear |
Morphotype | Siphoviridae |
Number of Genes | 84 |
Number of tRNAs | 3 |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK279903 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Not in Pitt Archives |
Available Files |
Plaque Picture | Download |
GenBank File for Phamerator | Download |