| Detailed Information for Phage Zetzy |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tessa Retzlaff |
| Year Found | 2013 |
| Location Found | Clinton, NE USA |
| Finding Institution | Chadron State College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.750243 N, 102.333481 W Map |
| Discovery Notes | The soil sample was collected about an inch deep in the middle of an alfalfa field. After a nice rain the night before. The time was at 9:16 in the morning at 26C. More than one phage was in this soil sample. |
| Naming Notes | The name for the phage is the nickname for my past twin brother, who always made fun of me for being a nerd. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 29, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 83 |
| Sanger Finishing Reactions | 6 |
| Genome length (bp) | 48229 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGTGGTAA |
| GC Content | 64.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | after 48hrs
diameter: 3mm
both turbid and clear |
| Morphotype | Siphoviridae |
| Number of Genes | 84 |
| Number of tRNAs | 3 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK279903 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
