| Detailed Information for Phage GardenState |
| Discovery Information |
| Isolation Host | Microbacterium sp. ISAT203 |
| Found By | Paulina Onisko |
| Year Found | 2019 |
| Location Found | Cranford, NJ United States |
| Finding Institution | James Madison University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.6584 N, 74.2996 W Map |
| Discovery Notes | This phage was discovered in the soil from New Jersey. It was discovered through being enriched with Microbacterium. After being enriched with Microbacterium and becoming evident on the Luria Broth Agar plate as a bacteriophage, the phage was picked, replicated, diluted through a dilution series, picked through passaging, and a full plate infection was done on it. |
| Naming Notes | The name of this phage came from the place and state it was discovered in. This phage was discovered in my backyard garden in New Jersey, which is often referred to as the "Garden State." |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1087 |
| Genome length (bp) | 45424 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGGGAGGCAT |
| GC Content | 70.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EH |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | James Madison University |
| Annotation Status | In GenBank |
| Plaque Notes | This plaque usually has large circular spots which are clear. The spots often have a large cloudy circular ring surrounding each one. |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT952845 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
172 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
