| Detailed Information for Phage GardenState | |
| Discovery Information | |
| Isolation Host | Microbacterium sp. ISAT203 |
| Found By | Paulina Onisko |
| Year Found | 2019 |
| Location Found | Cranford, NJ United States |
| Finding Institution | James Madison University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.6584 N, 74.2996 W Map |
| Discovery Notes | This phage was discovered in the soil from New Jersey. It was discovered through being enriched with Microbacterium. After being enriched with Microbacterium and becoming evident on the Luria Broth Agar plate as a bacteriophage, the phage was picked, replicated, diluted through a dilution series, picked through passaging, and a full plate infection was done on it. |
| Naming Notes | The name of this phage came from the place and state it was discovered in. This phage was discovered in my backyard garden in New Jersey, which is often referred to as the "Garden State." |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1087 |
| Genome length (bp) | 45424 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGGGAGGCAT |
| GC Content | 70.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EH |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to View |
| Annotating Institution | James Madison University |
| Annotation Status | In GenBank |
| Plaque Notes | This plaque usually has large circular spots which are clear. The spots often have a large cloudy circular ring surrounding each one. |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewGardenState_1 GardenState_2 GardenState_3 GardenState_4 GardenState_5 GardenState_6 GardenState_7 GardenState_8 GardenState_9 GardenState_10 GardenState_11 GardenState_12 GardenState_13 GardenState_14 GardenState_15 GardenState_16 GardenState_17 GardenState_18 GardenState_19 GardenState_20 GardenState_21 GardenState_22 GardenState_23 GardenState_24 GardenState_25 GardenState_26 GardenState_27 GardenState_28 GardenState_29 GardenState_30 GardenState_31 GardenState_32 GardenState_33 GardenState_34 GardenState_35 GardenState_36 GardenState_37 GardenState_38 GardenState_39 GardenState_40 GardenState_41 GardenState_42 GardenState_43 GardenState_44 GardenState_45 GardenState_46 GardenState_47 GardenState_48 GardenState_49 GardenState_50 GardenState_51 GardenState_52 GardenState_53 GardenState_54 GardenState_55 GardenState_56 GardenState_57 GardenState_58 GardenState_59 GardenState_60 GardenState_61 GardenState_62 GardenState_63 GardenState_64 GardenState_65 GardenState_66 GardenState_67 GardenState_68 GardenState_69 GardenState_70 GardenState_71 GardenState_72 GardenState_73 GardenState_74 GardenState_75 GardenState_76 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT952845 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 172 |
| Pitt Freezer Box Grid# | E3 |
| Available Files | |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
