| Detailed Information for Phage GodonK |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Lucy Liu |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.442958 N, 79.9565 W Map |
| Discovery Notes | Flower pot by the stairs of the Litchfield Towers on the right when facing toward the buildings
•Weather conditions at time of collection:
•Chilly and overcast
•43°F
•pot had contained no plants
•soil was very moist and it seems as though it had alot of metal (shiny) particles in it as well |
| Naming Notes | Mrs. Godon was my guidance councilor in high school who gave me endless of moral support, pushed me to believe in myself, and take on challenges which I would have never thought I could dream of. The K stands for Mrs. Krumm was the closest teacher, tutor, and mentor I had. She taught me so much and had worked with me for the longest time. She is the reason why I was able to excel in my studies and got me through so much back in my primary school years and middle school years. Without her persistence in helping me, I really would not have been able to do as well as I would have now. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 8, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1606 |
| Genome length (bp) | 122563 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 7581 bp |
| GC Content | 54.5% |
| Sequencing Notes | called start at base 1, about 50% of reads seem to start at base 2, PAUSE data agrees |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DK |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Indian River State College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques from the Direct Plating was very tiny. So tiny that it took 4 people to recognize that there were 3 plaques on the dish. |
| Morphotype | Siphoviridae |
| Number of Genes | 214 |
| Number of tRNAs | 31 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK620899 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
D7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
