| Detailed Information for Phage IdentityCrisis |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Justice B. Widman |
| Year Found | 2018 |
| Location Found | Port Saint Lucie, FL USA |
| Finding Institution | Indian River State College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 27.316112 N, 80.408922 W Map |
| Discovery Notes | Found under a plant bed near a local strip mall. Phage obtained during middle of day and had been collected in hot and dry day, with somewhat moist soil. |
| Naming Notes | Phage is named after the difficulty of obtaining a single isolated phage. During isolation step, the phage was unwilling to separate from another and made me waste 5 weeks trying to isolate without giving any indication that there was multiple phages present. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 27, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 332 |
| Genome length (bp) | 38341 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCAGGGCAT |
| GC Content | 65.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Indian River State College |
| Annotation Status | In GenBank |
| Number of Genes | 60 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN234184 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
77 |
| Pitt Freezer Box Grid# |
H11 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |