Mycobacterium phage IdentityCrisis
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Detailed Information for Phage IdentityCrisis
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJustice B. Widman
Year Found2018
Location FoundPort Saint Lucie, FL USA
Finding InstitutionIndian River State College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates27.316112 N, 80.408922 W Map
Discovery NotesFound under a plant bed near a local strip mall. Phage obtained during middle of day and had been collected in hot and dry day, with somewhat moist soil.
Naming NotesPhage is named after the difficulty of obtaining a single isolated phage. During isolation step, the phage was unwilling to separate from another and made me waste 5 weeks trying to isolate without giving any indication that there was multiple phages present.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 27, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage332
Genome length (bp)38341
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCTCAGGGCAT
GC Content65.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionIndian River State College
Annotation StatusBeing Annotated (Expected completion by 7/31/2019)
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 77
Pitt Freezer Box Grid# H11
Available Files
EM PictureDownload