| Detailed Information for Phage JustASigh |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Deborah Jacobs-Sera |
| Year Found | 2023 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.59485 N, 79.982255 W Map |
| Discovery Notes | Phage was isolated from 50x concentrated wastewater collected from MTSA-Pine-Creek-STP on 10/5/23. Samples were concentrated on 10/13/23 after filtering (.22um) using 30kDa Centricon® Plus-70 Filter Units by Jakobi D, and tested for phage activity by Nate W. & Sergei E.-D. |
| Naming Notes | This phage is named for David A. Asai.
"A kiss is just a kiss
A sigh is just a sigh
The fundamental things apply
As time goes by"
Lyrics of the song "As time goes by" by Dooley Wilson. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 811 |
| Genome length (bp) | 55871 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | TGCCGGCGCGGTA |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AH |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are tiny pinpoints. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP750961 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
