Arthrobacter phage AustinPowers
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Detailed Information for Phage AustinPowers
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found ByRutger Van Hees
Year Found2022
Location FoundRussellville, AR United States
Finding InstitutionBaylor University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature32°C
GPS Coordinates35.275 N, 93.153056 W Map
Discovery NotesSoil sample was retrieved ~5 inches below the surface in a garden. The soil sample was damp when collected.
Naming NotesNamed in honor of the best character ever played by Mike Meyers. Austin Powers is also my favorite movie series.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 30, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3021
Genome length (bp)43761
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceGCTTACCGTGATA
GC Content61.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAK
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionBaylor University
Annotation StatusQC Complete
Plaque NotesPlaques had clear, defined edges. Which indicates the bacteria was lysed by phages in the lytic lifecycle. Plaque sizes varied with sizes ranging from 1-2 mm.
The collected lysate had a titer of 8.0 x 10^10.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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AustinPowers_1

AustinPowers_2
(terminase)

AustinPowers_3
(portal protein)

AustinPowers_4
(capsid maturation protease)

AustinPowers_5
(major capsid protein)

AustinPowers_6

AustinPowers_7
(head-to-tail adaptor)

AustinPowers_8
(head-to-tail stopper)

AustinPowers_9

AustinPowers_10
(tail terminator)

AustinPowers_11
(major tail protein)

AustinPowers_12
(tail assembly chaperone)

AustinPowers_13
(tail assembly chaperone)

AustinPowers_14
(tape measure protein)

AustinPowers_15
(minor tail protein)

AustinPowers_16
(minor tail protein)

AustinPowers_17
(minor tail protein)

AustinPowers_18
(minor tail protein)

AustinPowers_19

AustinPowers_20

AustinPowers_21
(endolysin)

AustinPowers_22
(holin)

AustinPowers_23
(minor tail protein)

AustinPowers_24
(helix-turn-helix DNA binding domain)

AustinPowers_25

AustinPowers_26
(DNA helicase)

AustinPowers_27

AustinPowers_28

AustinPowers_29

AustinPowers_30

AustinPowers_31
(RuvC-like resolvase)

AustinPowers_32

AustinPowers_33

AustinPowers_34

AustinPowers_35

AustinPowers_36

AustinPowers_37

AustinPowers_38

AustinPowers_39
(Cas4 exonuclease)

AustinPowers_40

AustinPowers_41
(ASCE ATPase)

AustinPowers_42

AustinPowers_43
(DNA primase/polymerase/helicase)

AustinPowers_44

AustinPowers_45
(DNA polymerase I)

AustinPowers_46
(adenylosuccinate synthetase, PurA-like)

AustinPowers_47

AustinPowers_48

AustinPowers_49
(dUTPase)

AustinPowers_50

AustinPowers_51

AustinPowers_52

AustinPowers_53
(helix-turn-helix DNA binding domain)

AustinPowers_54

AustinPowers_55

AustinPowers_56

AustinPowers_57

AustinPowers_58

AustinPowers_59

AustinPowers_60
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 162
Pitt Freezer Box Grid# A8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload