Arthrobacter phage Karkharias
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Detailed Information for Phage Karkharias
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Former namesSharkesha
Found ByAllison Kenney and Vanessa Mleczko
Year Found2023
Location FoundDanvers, MA United States
Finding InstitutionMount Saint Mary College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates42.57045 N, 70.97622 W Map
Discovery NotesThis phage was discovered in front of a hotel from soil under a tree.
Naming NotesMy lab partner and I have a special bond formed through our love of sharks. This is so special to us that we had to incorporate it into our name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 17, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1270
Genome length (bp)42611
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGGAGA
GC Content65.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterFA
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionMount Saint Mary College
Annotation StatusIn GenBank
Number of Genes66
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Karkharias_1
(terminase, small subunit)

Karkharias_2
(terminase, large subunit)

Karkharias_3
(portal protein)

Karkharias_4
(capsid maturation protease)

Karkharias_5
(scaffolding protein)

Karkharias_6
(major capsid protein)

Karkharias_7
(capsid decoration protein)

Karkharias_8

Karkharias_9
(head-to-tail adaptor)

Karkharias_10
(head-to-tail stopper)

Karkharias_11

Karkharias_12
(tail terminator)

Karkharias_13

Karkharias_14
(major tail protein)

Karkharias_15
(tail assembly chaperone)

Karkharias_16
(tail assembly chaperone)

Karkharias_17

Karkharias_18
(tape measure protein)

Karkharias_19
(minor tail protein)

Karkharias_20
(minor tail protein)

Karkharias_21
(minor tail protein)

Karkharias_22
(minor tail protein)

Karkharias_23

Karkharias_24

Karkharias_25
(endolysin)

Karkharias_26

Karkharias_27

Karkharias_28

Karkharias_29

Karkharias_30
(esterase)

Karkharias_31

Karkharias_32
(esterase)

Karkharias_33

Karkharias_34
(tyrosine integrase)

Karkharias_35

Karkharias_36

Karkharias_37
(tyrosine integrase)

Karkharias_38
(helix-turn-helix DNA binding domain)

Karkharias_39
(helix-turn-helix DNA binding domain)

Karkharias_40
(helix-turn-helix DNA binding domain)

Karkharias_41

Karkharias_42

Karkharias_43

Karkharias_44

Karkharias_45

Karkharias_46
(Cas4 exonuclease)

Karkharias_47

Karkharias_48

Karkharias_49

Karkharias_50

Karkharias_51

Karkharias_52

Karkharias_53

Karkharias_54

Karkharias_55

Karkharias_56

Karkharias_57

Karkharias_58
(DNA primase/polymerase/helicase)

Karkharias_59

Karkharias_60

Karkharias_61

Karkharias_62
(Holliday junction resolvase)

Karkharias_63

Karkharias_64

Karkharias_65

Karkharias_66
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ184797
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 3.24 x1010 PFU/ml
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload