| Detailed Information for Phage Kukla | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Steven Siegel |
| Year Found | 2023 |
| Location Found | Richmond, IL United States |
| Finding Institution | Baylor University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.47707 N, 88.31181 W Map |
| Discovery Notes | The phage was found 3-5 inches below the soil surface. It rained two days prior so the soil was still damp. It was determined that the soil type was sandy loam (71.43% sand, 25.71% silt, and 2.86% clay) with a pH of 5.5. |
| Naming Notes | The last name Kukla is from the owner of the house where I collected my soil/ phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1017 |
| Genome length (bp) | 46697 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FJ |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | Baylor University |
| Annotation Status | In GenBank |
| Plaque Notes | The average plaque size of the phage is 1.53mm. The type of virus is a Siphoviridae with an average tail length of 185nm and and average head diameter of 51.5nm. |
| Morphotype | Siphoviridae |
| Number of Genes | 84 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewKukla_1 Kukla_2 Kukla_3 Kukla_4 Kukla_5 Kukla_6 Kukla_7 Kukla_8 Kukla_9 Kukla_10 Kukla_11 Kukla_12 Kukla_13 Kukla_14 Kukla_15 Kukla_16 Kukla_17 Kukla_18 Kukla_19 Kukla_20 Kukla_21 Kukla_22 Kukla_23 Kukla_24 Kukla_25 Kukla_26 Kukla_27 Kukla_28 Kukla_29 Kukla_30 Kukla_31 Kukla_32 Kukla_33 Kukla_34 Kukla_35 Kukla_36 Kukla_37 Kukla_38 Kukla_39 Kukla_40 Kukla_41 Kukla_42 Kukla_43 Kukla_44 Kukla_45 Kukla_46 Kukla_47 Kukla_48 Kukla_49 Kukla_50 Kukla_51 Kukla_52 Kukla_53 Kukla_54 Kukla_55 Kukla_56 Kukla_57 Kukla_58 Kukla_59 Kukla_60 Kukla_61 Kukla_62 Kukla_63 Kukla_64 Kukla_65 Kukla_66 Kukla_67 Kukla_68 Kukla_69 Kukla_70 Kukla_71 Kukla_72 Kukla_73 Kukla_74 Kukla_75 Kukla_76 Kukla_77 Kukla_78 Kukla_79 Kukla_80 Kukla_81 Kukla_82 Kukla_83 Kukla_84 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ362669 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 184 |
| Pitt Freezer Box Grid# | H12 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
