Arthrobacter phage Kukla
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Detailed Information for Phage Kukla
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found BySteven Siegel
Year Found2023
Location FoundRichmond, IL United States
Finding InstitutionBaylor University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature32°C
GPS Coordinates42.47707 N, 88.31181 W Map
Discovery NotesThe phage was found 3-5 inches below the soil surface. It rained two days prior so the soil was still damp. It was determined that the soil type was sandy loam (71.43% sand, 25.71% silt, and 2.86% clay) with a pH of 5.5.
Naming NotesThe last name Kukla is from the owner of the house where I collected my soil/ phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1017
Genome length (bp)46697
Character of genome endsCircularly Permuted
GC Content64.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterFJ
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members

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Annotating InstitutionBaylor University
Annotation StatusIn GenBank
Plaque NotesThe average plaque size of the phage is 1.53mm.
The type of virus is a Siphoviridae with an average tail length of 185nm and and average head diameter of 51.5nm.
MorphotypeSiphoviridae
Number of Genes84
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Kukla_1
(terminase)

Kukla_2
(portal protein)

Kukla_3

Kukla_4

Kukla_5
(capsid maturation protease)

Kukla_6

Kukla_7
(major capsid protein)

Kukla_8

Kukla_9

Kukla_10

Kukla_11
(tail terminator)

Kukla_12

Kukla_13
(major tail protein)

Kukla_14
(tail assembly chaperone)

Kukla_15
(tail assembly chaperone)

Kukla_16

Kukla_17
(tape measure protein)

Kukla_18
(minor tail protein)

Kukla_19
(minor tail protein)

Kukla_20

Kukla_21
(minor tail protein)

Kukla_22
(endolysin)

Kukla_23

Kukla_24
(minor tail protein)

Kukla_25
(minor tail protein)

Kukla_26

Kukla_27

Kukla_28

Kukla_29
(hydrolase)

Kukla_30
(hydrolase)

Kukla_31
(acetyltransferase)

Kukla_32

Kukla_33
(helix-turn-helix DNA binding domain protein)

Kukla_34

Kukla_35

Kukla_36
(tyrosine integrase)

Kukla_37
(helix-turn-helix DNA binding domain protein)

Kukla_38
(metalloprotease)

Kukla_39
(helix-turn-helix DNA binding domain protein)

Kukla_40
(helix-turn-helix DNA binding domain protein)

Kukla_41
(helix-turn-helix DNA binding domain protein)

Kukla_42

Kukla_43
(phosphatase)

Kukla_44

Kukla_45

Kukla_46
(helix-turn-helix DNA binding domain protein)

Kukla_47
(DNA binding protein)

Kukla_48

Kukla_49

Kukla_50

Kukla_51

Kukla_52
(RecE-like exonuclease)

Kukla_53
(RecT-like DNA pairing protein)

Kukla_54

Kukla_55
(SSB protein)

Kukla_56
(NrdH-like glutaredoxin)

Kukla_57

Kukla_58
(helix-turn-helix DNA binding domain protein)

Kukla_59
(helix-turn-helix DNA binding domain protein)

Kukla_60

Kukla_61

Kukla_62

Kukla_63
(nuclease)

Kukla_64

Kukla_65

Kukla_66
(Holliday junction resolvase)

Kukla_67

Kukla_68

Kukla_69

Kukla_70

Kukla_71

Kukla_72

Kukla_73

Kukla_74

Kukla_75

Kukla_76

Kukla_77

Kukla_78

Kukla_79

Kukla_80

Kukla_81

Kukla_82

Kukla_83

Kukla_84

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ362669
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 184
Pitt Freezer Box Grid# H12
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload