| Detailed Information for Phage Molivia | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Alexandria F. Martinez |
| Year Found | 2015 |
| Location Found | Radnor, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.3475 N, 76.006944 W Map |
| Discovery Notes | The soil sample was collected from a small, muddy creek bank directly across from Township of Spring Waste Water Treatment Facility, near Cacoosing Meadows Recreation Park in Sinking Spring, PA. The enrichment process revealed small, aquatic organisms in the soil. |
| Naming Notes | This phage name is a name mesh dedicated to my niece and nephew, Moises and Olivia. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 536 |
| Genome length (bp) | 58247 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1467 bp |
| End Determination Notes | One end (the right) is very clear, while the other end (left) is more ambiguous. This is consistent with other Cluster AQ phages. |
| GC Content | 53.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AQ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Nature of the phage consists of three plaque sizes: significantly small, mid-sized, and large plaques. Molivia also produces a consistent web pattern at T-3 with 25mM CaCl2 top agar and an overnight incubation at 30 degrees Celsius. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 6 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMolivia_1 Molivia_2 Molivia_3 Molivia_4 Molivia_5 Molivia_6 Molivia_7 Molivia_8 Molivia_9 Molivia_10 Molivia_11 Molivia_12 Molivia_13 Molivia_14 Molivia_15 Molivia_16 Molivia_17 Molivia_18 — tRNA Molivia_19 — tRNA Molivia_20 — tRNA Molivia_21 — tRNA Molivia_22 Molivia_23 Molivia_24 Molivia_25 Molivia_26 Molivia_27 Molivia_28 Molivia_29 Molivia_30 Molivia_31 Molivia_32 Molivia_33 Molivia_34 Molivia_35 Molivia_36 Molivia_37 Molivia_38 Molivia_39 Molivia_40 Molivia_41 Molivia_42 Molivia_43 Molivia_44 Molivia_45 Molivia_46 Molivia_47 Molivia_48 Molivia_49 Molivia_50 Molivia_51 Molivia_52 Molivia_53 Molivia_54 Molivia_55 Molivia_56 Molivia_57 Molivia_58 Molivia_59 — tRNA Molivia_60 — tRNA Molivia_61 Molivia_62 Molivia_63 Molivia_64 Molivia_65 Molivia_66 Molivia_67 Molivia_68 Molivia_69 Molivia_70 Molivia_71 Molivia_72 Molivia_73 Molivia_74 Molivia_75 Molivia_76 Molivia_77 Molivia_78 Molivia_79 Molivia_80 Molivia_81 Molivia_82 Molivia_83 Molivia_84 Molivia_85 Molivia_86 Molivia_87 Molivia_88 Molivia_89 Molivia_90 Molivia_91 Molivia_92 Molivia_93 Molivia_94 Molivia_95 Molivia_96 Molivia_97 Molivia_98 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF185731 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | Cabrini College |
| SEA Lysate Titer | 5.0e9 pfu/mL |
| Date of SEA Lysate Titering | Jun 24, 2015 |
| Pitt Freezer Box# | 149 |
| Pitt Freezer Box Grid# | C10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |