Arthrobacter phage Nahla
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Duplicate of Piccoletto
After further review, this phage turned out to be 100% identical to Piccoletto, another phage found at UTEP in the same time frame.
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| Detailed Information for Phage Nahla | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Ana Alvidrez and Kennedy Behrens |
| Year Found | 2015 |
| Location Found | Cloudcroft, NM USA |
| Finding Institution | University of Texas at El Paso |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.829367 N, 105.739117 W Map |
| Discovery Notes | This is the same soil sample used to isolate Arthrobacteriophage Piccoletto. The sample was collected on a cloudy day at around 1:00 PM. The elevation of the place where the sample was collected is 2,518 m above sea level. The sample was collected at the root of a mature pine. |
| Naming Notes | Nahla is the name of one of the characters in Disney's "The Lion King." The students considered that it was worth giving the phage that name because it represents courage and persistence but with a female personality. It took substantial persistence to isolate this phage - a total of 51 different samples were collectively analyzed by our Phage Hunters fall 2015 class, 9 collected in Cloudcroft, NM, and 42 collected in El Paso, TX. Out of all those only this one sample turned out phages. Interestingly, the name of the character in "The Lion King" is spelled "Nala", without the "h". |
| Sequencing Information | |
| Sequencing Complete? | No |
| Date Sequencing Completed | Jan 29, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2664 |
| Genome length (bp) | 49869 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.5% |
| Fasta file available? | No |
| Characterization | |
| Cluster | AO |
| Subcluster | AO1 |
| Cluster Life Cycle | Lytic |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Nahla produces relatively large clear plaques, of about 3.175 mm in diameter, when grown in an Arthrobacter sp. host at 35oC for about 16-18 hours. |
| Morphotype | Myoviridae |
| Has been Phamerated? | No |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 26 |
| Pitt Freezer Box Grid# | F7 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
