Microbacterium phage Rasputia
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| Detailed Information for Phage Rasputia | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Jelsia Cottone and Gabrielle Berwick |
| Year Found | 2019 |
| Location Found | Langhorne, PA USA |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 40.160561 N, 75.002129 W Map |
| Discovery Notes | Found in a garden containing moist dark brown colored soil. Close to homes and main roads. With lots of foot trafficking done by a cute dog. |
| Naming Notes | The name of this phage is Rasputia. It came from a movie that we believe fit the characteristics of our phage when we saw the EM screening. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 20, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 47 |
| Genome length (bp) | 97105 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.3% |
| Sequencing Notes | The phage was sequenced as part of a DOGEMS sample. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | GC |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | High lysate titer was calculated to 8.3 X 10^8 pfu/mL. |
| Other Cluster Members | |
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | It appears to be a lytic life cycle, because the plaques edges are small, clear and circular. The plaque sizes are 1.42 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 153 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT639646 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 8.3 x 10^8 |
| Pitt Freezer Box# | 98 |
| Pitt Freezer Box Grid# | D10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |