| Detailed Information for Phage Rooney |
| Discovery Information |
| Isolation Host | Streptomyces viridochromogenes DSM40736 |
| Found By | Hannah Boozalis and Margaret Gaggioli |
| Year Found | 2015 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 29°C |
| GPS Coordinates | 38.648 N, 90.306 W Map |
| Discovery Notes | The sample was taken from a muddy puddle next to a path in Brookings Quad on the Danforth Campus of Washington University in St. Louis. |
| Naming Notes | 'Rooney' comes from the name of the Dean of Students in "Ferris Bueller's Day Off". |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 20, 2020 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 165 |
| Genome length (bp) | 69514 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were relatively small in size and mostly circular in shape. They were clear and had defined edges. After purification they had an average radius of 1 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 105 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ648033 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Designator |
Rooney |
| SEA Lysate Titer |
1.43x10^10 pfu/ml |
| Date of SEA Lysate Titering |
Oct 8, 2015 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
