| Detailed Information for Phage RosiePosie | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Rosendo Villafuerte-Vega |
| Year Found | 2015 |
| Location Found | Immaculata, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.029722 N, 75.574167 W Map |
| Discovery Notes | Soil sample acquired behind the West Campus Apartments of Immaculata University. Soil was moderately moist. |
| Naming Notes | People have a difficult time remembering my name, so they often resort to calling me "Rosie Posie." So, why not name a phage that has some relevance to my name? It makes it more personable! |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 668 |
| Genome length (bp) | 70396 |
| Character of genome ends | Circularly Permuted |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | RosiePosie forms small, clear plaques. Plaque size remained consistent during the isolation process. Low titer. Optimal incubation temperature between room temperature and 30 degrees Celsius. Grew well without CaCl2, as it did with 5mM and 50mM CaCl2; however, it did not grow at 37 degrees Celsius regardless of varying CaCl2 concentrations. RosiePosie also grew well at MgSO4 concentrations of 5, 25, and 50mM concentrations when incubated at room temperature and 30 degrees Celsius. Interestingly, a clear cluster of plaques was evident for all concentrations of MgSO4 at 37 degrees Celsius for an undiluted sample of high titer lysate. In the end, plaques were clearer in the presence of magnesium. |
| Morphotype | Myoviridae |
| Number of Genes | 112 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewRosiePosie_1 RosiePosie_2 RosiePosie_3 RosiePosie_4 RosiePosie_5 RosiePosie_6 RosiePosie_7 RosiePosie_8 RosiePosie_9 RosiePosie_10 RosiePosie_11 RosiePosie_12 RosiePosie_13 RosiePosie_14 RosiePosie_15 RosiePosie_16 RosiePosie_17 RosiePosie_18 RosiePosie_19 RosiePosie_20 RosiePosie_21 RosiePosie_22 RosiePosie_23 RosiePosie_24 RosiePosie_25 RosiePosie_26 RosiePosie_27 RosiePosie_28 RosiePosie_29 RosiePosie_30 RosiePosie_31 RosiePosie_32 RosiePosie_33 RosiePosie_34 RosiePosie_35 RosiePosie_36 RosiePosie_37 RosiePosie_38 RosiePosie_39 RosiePosie_40 RosiePosie_41 RosiePosie_42 RosiePosie_43 RosiePosie_44 RosiePosie_45 RosiePosie_46 RosiePosie_47 RosiePosie_48 RosiePosie_49 RosiePosie_50 RosiePosie_51 RosiePosie_52 RosiePosie_53 RosiePosie_54 RosiePosie_55 RosiePosie_56 RosiePosie_57 RosiePosie_58 RosiePosie_59 RosiePosie_60 RosiePosie_61 RosiePosie_62 RosiePosie_63 RosiePosie_64 RosiePosie_65 RosiePosie_66 RosiePosie_67 RosiePosie_68 RosiePosie_69 RosiePosie_70 RosiePosie_71 RosiePosie_72 RosiePosie_73 RosiePosie_74 RosiePosie_75 RosiePosie_76 RosiePosie_77 RosiePosie_78 RosiePosie_79 RosiePosie_80 RosiePosie_81 RosiePosie_82 RosiePosie_83 RosiePosie_84 RosiePosie_85 RosiePosie_86 RosiePosie_87 RosiePosie_88 RosiePosie_89 RosiePosie_90 RosiePosie_91 RosiePosie_92 RosiePosie_93 RosiePosie_94 RosiePosie_95 RosiePosie_96 RosiePosie_97 RosiePosie_98 RosiePosie_99 RosiePosie_100 RosiePosie_101 RosiePosie_102 RosiePosie_103 RosiePosie_104 RosiePosie_105 RosiePosie_106 RosiePosie_107 RosiePosie_108 RosiePosie_109 RosiePosie_110 RosiePosie_111 RosiePosie_112 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF185723 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | Cabrini College |
| SEA Lysate Titer | 6.52 x 10^8 pfu/mL |
| Date of SEA Lysate Titering | Jul 15, 2015 |
| Pitt Freezer Box# | 149 |
| Pitt Freezer Box Grid# | D4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
