Arthrobacter phage Sakai
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Detailed Information for Phage Sakai
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByBrady Johnson
Year Found2018
Location FoundRiver Falls, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature28°C
GPS Coordinates44.8523 N, 92.6153 W Map
Discovery NotesThis phage was originally co-isolated with Gorpy. The two phages were separated by Scott Christensen and Vincent Kromrey.
Naming NotesNamed for a character from a favorite video game
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 13, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage176
Genome length (bp)52271
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGGTGA
GC Content62.7%
Sequencing NotesWhen this phage was sequenced, it turned out to be mixed with a similar phage. They were eventually separated both bioinformatically and at the bench, and so both sequences were posted. The other phage is Gorpy.
Fasta file available?Yes: Download fasta file
Characterization
ClusterAY
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusIn GenBank
Plaque NotesClear plaques with variable size, 0.5-2 mm.
MorphotypeSiphoviridae
Number of Genes92
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Sakai_1
(terminase small subunit)

Sakai_2
(terminase large subunit, ATPase domain)

Sakai_3
(terminase large subunit, nuclease domain)

Sakai_4

Sakai_5
(portal protein)

Sakai_6
(scaffolding protein)

Sakai_7
(LamD-like capsid decoration protein)

Sakai_8
(major capsid protein)

Sakai_9

Sakai_10
(head-to-tail adaptor)

Sakai_11
(head-to-tail stopper)

Sakai_12

Sakai_13
(tail terminator)

Sakai_14

Sakai_15
(major tail protein)

Sakai_16
(tail assembly chaperone)

Sakai_17
(tail assembly chaperone)

Sakai_18

Sakai_19
(tape measure protein)

Sakai_20
(minor tail protein)

Sakai_21
(minor tail protein)

Sakai_22
(minor tail protein)

Sakai_23
(minor tail protein)

Sakai_24

Sakai_25

Sakai_26

Sakai_27
(endolysin)

Sakai_28

Sakai_29

Sakai_30

Sakai_31

Sakai_32
(esterase)

Sakai_33

Sakai_34
(lipase)

Sakai_35

Sakai_36
(tyrosine integrase)

Sakai_37

Sakai_38

Sakai_39
(helix-turn-helix DNA binding protein)

Sakai_40
(tyrosine integrase)

Sakai_41

Sakai_42

Sakai_43

Sakai_44

Sakai_45

Sakai_46

Sakai_47

Sakai_48

Sakai_49
(histidine triad nucleotide binding protein)

Sakai_50
(helix-turn-helix DNA binding protein)

Sakai_51
(helix-turn-helix DNA binding protein)

Sakai_52
(helix-turn-helix DNA binding protein)

Sakai_53
(helix-turn-helix DNA binding protein)

Sakai_54

Sakai_55
(DNA binding protein)

Sakai_56

Sakai_57

Sakai_58

Sakai_59

Sakai_60

Sakai_61

Sakai_62

Sakai_63

Sakai_64

Sakai_65

Sakai_66
(exonuclease)

Sakai_67

Sakai_68
(AAA-ATPase)

Sakai_69
(ssDNA binding protein)

Sakai_70

Sakai_71
(thioredoxin)

Sakai_72
(DNA helicase/methylase)

Sakai_73
(helix-turn-helix DNA binding protein)

Sakai_74
(DNA polymerase III sliding clamp beta)

Sakai_75

Sakai_76
(DNA methylase)

Sakai_77

Sakai_78

Sakai_79

Sakai_80

Sakai_81
(MazG-like pyrophosphohydrolase)

Sakai_82
(RusA-like resolvase)

Sakai_83

Sakai_84

Sakai_85

Sakai_86

Sakai_87

Sakai_88
(helix-turn-helix DNA binding protein)

Sakai_89

Sakai_90

Sakai_91
(metalloprotease)

Sakai_92

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON970598
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 140
Pitt Freezer Box Grid# A12
Available Files
GenBank File for PhameratorDownload