| Detailed Information for Phage Sakai |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Brady Johnson |
| Year Found | 2018 |
| Location Found | River Falls, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.8523 N, 92.6153 W Map |
| Discovery Notes | This phage was originally co-isolated with Gorpy. The two phages were separated by Scott Christensen and Vincent Kromrey. |
| Naming Notes | Named for a character from a favorite video game |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 13, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 176 |
| Genome length (bp) | 52271 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGTGA |
| GC Content | 62.7% |
| Sequencing Notes | When this phage was sequenced, it turned out to be mixed with a similar phage. They were eventually separated both bioinformatically and at the bench, and so both sequences were posted. The other phage is Gorpy. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AY |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques with variable size, 0.5-2 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970598 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
140 |
| Pitt Freezer Box Grid# |
A12 |
| Available Files |
| GenBank File for Phamerator | Download |