Detailed Information for Phage Sakai |
Discovery Information |
Isolation Host | Arthrobacter globiformis B-2979 |
Found By | Brady Johnson |
Year Found | 2018 |
Location Found | River Falls, WI USA |
Finding Institution | University of Wisconsin-River Falls |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Unknown |
Isolation Temperature | 28°C |
GPS Coordinates | 44.8523 N, 92.6153 W Map |
Discovery Notes | This phage was originally co-isolated with Gorpy. The two phages were separated by Scott Christensen and Vincent Kromrey. |
Naming Notes | Named for a character from a favorite video game |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Dec 13, 2021 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 176 |
Genome length (bp) | 52271 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 9 bases |
Overhang Sequence | CGCCGGTGA |
GC Content | 62.7% |
Sequencing Notes | When this phage was sequenced, it turned out to be mixed with a similar phage. They were eventually separated both bioinformatically and at the bench, and so both sequences were posted. The other phage is Gorpy. |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | AY |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | University of Wisconsin-River Falls |
Annotation Status | In GenBank |
Plaque Notes | Clear plaques with variable size, 0.5-2 mm. |
Morphotype | Siphoviridae |
Number of Genes | 92 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | ON970598 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
140 |
Pitt Freezer Box Grid# |
A12 |
Available Files |
GenBank File for Phamerator | Download |