| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Ethan Edwards |
| Year Found | 2019 |
| Location Found | Manchester, NH United States |
| Finding Institution | University of New Hampshire at Manchester |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 18°C |
| GPS Coordinates | 42.994046 N, 71.512123 W Map |
| Discovery Notes | Location Descriptors:
- Backyard of a suburban household
- Area sampled from is was previously a plant grow bed and before that there was a sand pit above
Soil Descriptors:
- Approximately 60% sandy soil and 40% coarse soil
- Slightly moist, as it was in the morning and dew was fresh on the ground
- Flakey |
| Naming Notes | Due to difficulties getting the phage to represent itself on the bacterial lawn (personal error), and the practice of using phage to treat bacterial infections in both animals and humans.
I thought it appropriate to name the phage SilentRX. As it could be administered as "the Silent treatment." |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1301 |
| Genome length (bp) | 65232 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 838 bp |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AP |
| Subcluster | AP4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of New Hampshire at Manchester |
| Annotation Status | In GenBank |
| Plaque Notes | Size (Range): 0.2 mm to 1.2 mm [clear]
Details: 0.1 mm halo [turbid]
Other Notes: Despite range in sizes which only increased after purification, the morphology aside stayed very constant through all stages of processing. |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW862992 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.11x10^10 pfu/ml |
| Pitt Freezer Box# |
116 |
| Pitt Freezer Box Grid# |
B10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
