| Detailed Information for Phage SorJuana | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Trevor Cross |
| Year Found | 2014 |
| Location Found | Royersford, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.209448 N, 75.559227 W Map |
| Discovery Notes | Found during the winter under a section of frozen water from the Schuylkill River. Please note that phage lysate was made with glycerol for archiving purposes. |
| Naming Notes | Sor Juana was a nun during the 17th century in colonial Mexico who was known for her poetry and intellectual prowess. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2014 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 348 |
| Genome length (bp) | 58979 |
| Character of genome ends | Direct Terminal Repeat |
| GC Content | 53.0% |
| Sequencing Notes | This phage is Amigo-like, and thus has the same pattern of ends. There's a terminal redundancy where one end is clearly defined (the right end as the genome is canonically oriented) and the other end is less clear. The left end was chosen to match Amigo's left end, and also matches the approximate boundary of the region of double coverage in the SorJuana sequencing data. With the chosen end, the terminal repeat is 1589 bp, though this is approximate. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AQ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | 5 mm mostly clear plaques after 24 hours at 30⁰C on 4.5 mM CaCl2 |
| Morphotype | Siphoviridae |
| Number of Genes | 86 |
| Number of tRNAs | 7 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSorJuana_1 SorJuana_2 SorJuana_3 SorJuana_4 SorJuana_5 SorJuana_6 SorJuana_7 SorJuana_8 SorJuana_9 SorJuana_10 SorJuana_11 SorJuana_12 — tRNA SorJuana_13 — tRNA SorJuana_14 — tRNA SorJuana_15 — tRNA SorJuana_16 — tRNA SorJuana_17 SorJuana_18 SorJuana_19 SorJuana_20 SorJuana_21 SorJuana_22 SorJuana_23 SorJuana_24 SorJuana_25 SorJuana_26 SorJuana_27 SorJuana_28 SorJuana_29 SorJuana_30 SorJuana_31 SorJuana_32 SorJuana_33 SorJuana_34 SorJuana_35 SorJuana_36 SorJuana_37 SorJuana_38 SorJuana_39 SorJuana_40 SorJuana_41 SorJuana_42 SorJuana_43 SorJuana_44 SorJuana_45 SorJuana_46 SorJuana_47 SorJuana_48 SorJuana_49 SorJuana_50 SorJuana_51 SorJuana_52 SorJuana_53 SorJuana_54 SorJuana_55 SorJuana_56 SorJuana_57 — tRNA SorJuana_58 — tRNA SorJuana_59 SorJuana_60 SorJuana_61 SorJuana_62 SorJuana_63 SorJuana_64 SorJuana_65 SorJuana_66 SorJuana_67 SorJuana_68 SorJuana_69 SorJuana_70 SorJuana_71 SorJuana_72 SorJuana_73 SorJuana_74 SorJuana_75 SorJuana_76 SorJuana_77 SorJuana_78 SorJuana_79 SorJuana_80 SorJuana_81 SorJuana_82 SorJuana_83 SorJuana_84 SorJuana_85 SorJuana_86 SorJuana_87 SorJuana_88 SorJuana_89 SorJuana_90 SorJuana_91 SorJuana_92 SorJuana_93 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU160666 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 149 |
| Pitt Freezer Box Grid# | B4 |
| Available Files | |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |