The Actinobacteriophage Database

Mycobacterium phage Sparky

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Detailed Information for Phage Sparky
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Kassie Simmons-Yager
Year Found	2009
Location Found	Corvallis, OR USA
Finding Institution	Oregon State University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	44.562919 N, 123.274203 W Map
Discovery Notes	Moist dark top soil under drain drip spot. Same location and enrichment as 2009ORSUdarthphagerKT
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Aug 25, 2011
Genome length (bp)	63334
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGATGGCAT
GC Content	65.2%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	AF
Subcluster	--
Cluster Life Cycle	Unknown
Other Cluster Members	Click to View Farewell Sparky
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Clear plaques, 1/4 mm in diameter grows in 24hrs incubation temperature
Morphotype	Siphoviridae
Number of Genes	91
Number of tRNAs	4
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Sparky_1 Sparky_2 (terminase) Sparky_3 (portal protein) Sparky_4 (capsid maturation protease) Sparky_5 (scaffolding protein) Sparky_6 (major capsid protein) Sparky_7 (head-to-tail connector) Sparky_8 (head-to-tail stopper) Sparky_9 (head-to-tail connector) Sparky_10 (head-to-tail connector) Sparky_11 (major tail subunit) Sparky_12 (tail assembly chaperone) Sparky_13 (tail assembly chaperone) Sparky_14 (tape measure protein) Sparky_15 (minor tail protein) Sparky_16 (minor tail protein) Sparky_17 (minor tail protein) Sparky_18 (minor tail protein) Sparky_19 (minor tail protein) Sparky_20 Sparky_21 Sparky_22 Sparky_23 Sparky_24 Sparky_25 Sparky_26 (lysin A) Sparky_27 (lysin B) Sparky_28 Sparky_29 Sparky_30 Sparky_31 — tRNA (tRNA-Ser (gct)) Sparky_32 — tRNA (tRNA-Pro(tgg)) Sparky_33 — tRNA (tRNA-Trp(cca)) Sparky_34 — tRNA (tRNA-Tyr(gta)) Sparky_35 Sparky_36 (Ro-like RNA binding protein) Sparky_37 Sparky_38 Sparky_39 Sparky_40 Sparky_41 Sparky_42 Sparky_43 Sparky_44 Sparky_45 (integrase) Sparky_46 Sparky_47 (immunity repressor) Sparky_48 (CRO protein) Sparky_49 (HTH DNA binding protein) Sparky_50 Sparky_51 Sparky_52 Sparky_53 Sparky_54 Sparky_55 (DnaQ-like DNA polymerase III subunit) Sparky_56 (RecE) Sparky_57 (putative RecT) Sparky_58 Sparky_59 Sparky_60 Sparky_61 Sparky_62 Sparky_63 Sparky_64 (DNA methylase) Sparky_65 Sparky_66 (DNA helicase/methylase) Sparky_67 Sparky_68 Sparky_69 Sparky_70 Sparky_71 Sparky_72 Sparky_73 Sparky_74 Sparky_75 (WhiB) Sparky_76 Sparky_77 Sparky_78 Sparky_79 Sparky_80 (DnaQ-like exonuclease) Sparky_81 Sparky_82 Sparky_83 Sparky_84 Sparky_85 Sparky_86 Sparky_87 Sparky_88 (glycosyltransferase) Sparky_89 (glycosyltransferase) Sparky_90 (antitoxin) Sparky_91 (toxin) Sparky_92 Sparky_93 Sparky_94 Sparky_95 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KM083128
Refseq Number	NC_026597
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2009ORSUsparkyKSY
SEA Lysate Titer	5.8e9
Date of SEA Lysate Titering	Nov 13, 2009
Pitt Freezer Box#	5
Pitt Freezer Box Grid#	51
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download

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