| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Makayla Mobeck |
| Year Found | 2019 |
| Location Found | River Falls, WI United States of America |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.850147 N, 92.624343 W Map |
| Discovery Notes | Collected: September 4th, 2019
Time Collected: 5:20 PM
Location: On the amphitheater side between the amphitheater and river bridge. Under a big tree that is separate from the forest.
Soil Condition: Moist, packed down but easily loosened
Soil depth: ~76.2 mm (Collected from surface dirt to 76.2 mm below ground)
Air Temperature: 69 degrees Fahrenheit
Weather: Slightly cloudy, mostly clear. Cool breeze. |
| Naming Notes | Tokki is Korean for Bunny. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 8, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 54 |
| Genome length (bp) | 57652 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGCCC |
| GC Content | 50.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AU |
| Subcluster | AU2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of North Carolina at Charlotte |
| Annotation Status | In GenBank |
| Plaque Notes | Fairly clear plaques, ~1 mm plaques, very small. No halos and all uniform. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OK310503 |
| Refseq Number | None yet |
| Published in Paper | Aguilera Marti et al 2022, Microbiology Resource Announcements: Genome Sequence and Annotation of Bacteriophage Tokki Isolated on Arthrobacter globiformis B-2979 |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
102 |
| Pitt Freezer Box Grid# |
H8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
