Toolbox: Protocols and Information
Toolbox
The Toolbox was created to house those procedures that can be used in various parts of the workflow.| Protocol Title | Description/Goal |
|---|---|
| Using a Gel-Doc Camera to Take Plaque Pictures Details Download pdf |
To keep a visual record of plaque morphology using a GelDoc XR+ System. |
| Notebooks Details Download pdf |
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| Measuring DNA Concentration by NanoDrop Details Download pdf |
To quantify the amount of DNA in a phage or genomic DNA sample. |
| Picking a Plaque Details Download pdf |
To obtain a liquid sample of phage from a plaque (or putative plaque) on a lawn of bacteria. |
| Making Serial Dilutions Details Download pdf |
Create standard dilutions of a phage sample in order to perform plaque assays or titers. |
| Taking Plaque Pictures Details Download pdf |
To keep a visual record of findings. |
| Titering Details Download pdf |
To quantify the number of phage in a sample expressed as PFU (plaque forming unit)/mL. |
| Controls Details Download pdf |
To ascertain whether the plaque detection system is working. |
| Obtaining GPS Coordinates Details Download pdf |
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| Making Thumbnails for PhagesDB Details Download pdf |
Describes how to select, crop, and prepare good pictures for use as thumbnail images on PhagesDB.org. |
| Checking phage lysates by PCR Details Download pdf |
PCR verification is used to provide evidence that the phage lysate you are working with is the one that you want. Read the Helpful Tips to understand its limitations. A list of cluster- or phage- specific primer sets is found here. |
| Archive Protocols Details Download pdf |
If you submit information about a phage to PhagesDB.org, please submit a sample of the lysate to our archive inventory. Archiving includes sending a sample for our inventory AND making sure the info at PhagesDB is complete and accurate. If you are a member of the SEA-PHAGES program, the protocol for those sample preps are found here. If you are not a member of SEA-PHAGES, please consider submitting a sample and keeping your phage data up-to-date. contact Emily Ginser (emg190@pitt.edu) for additional information. A video is linked in the protocol for quick viewing! Also check out Archiving FAQs. https://phagesdb.org/protocols/119/ Protocol last modified 10-5-23. Shipping address is found in this protocol. Please check. |
| Archiving FAQs Details Download pdf |
Additional information when archiving lysates for SEA-PHAGES. Remember to make sure the phage page is complete and accurate. Updated 10-5-2023. |
| Requesting Phages Details Download pdf |
SEA-PHAGES phages can be requested for investigations by sending an email to Deborah Jacobs-Sera (djs@pitt.edu) on behalf of Dr. Graham Hatfull. |