Arthrobacter phage Anekin
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Detailed Information for Phage Anekin
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByLesley Avila
Year Found2022
Location FoundNaperville, IL United States
Finding InstitutionBenedictine University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature22°C
GPS Coordinates41.71679 N, 88.20451 W Map
Discovery NotesI discovered Anekin from a potted plant outside the Arbor Terrace facility. The weather was hot with clear skies and was primarily sunny all day. Some of the soil that was gathered was moist due to sprinklers that were on throughout the day. However, some of the soil collected had already dried out due to the weather conditions and timing of the collection. I used a fork and dug 2 and ½ inches before collecting about ½ a sandwich Ziploc bag of soil. The soil contained some dried plant leaves, minimal rocks, and roots
Naming NotesThe name "Anekin" was inspired by the Star Wars series. When this project started I was really into binge-watching the series.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 20, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage342
Genome length (bp)53469
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGGTGA
GC Content62.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAY
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionOregon Coast Community College
Annotation StatusAwaiting_QC
Plaque NotesThe plaques appear medium size and are consistently circular in form/figure. The plaques measure 2mm in diameter and have a radius of 1mm. They appear to be clear. The plaque characteristics suggest that this is a lytic phage.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload