| Discovery Information |
| Isolation Host | Rhodococcus erythropolis RIA 643 |
| Found By | Mitchell Broadway, Sydnie Gates |
| Year Found | 2015 |
| Location Found | Somerville, TN USA |
| Finding Institution | University of Louisiana at Monroe |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 34°C |
| GPS Coordinates | 35.243700 N, 89.350100 W Map |
| Discovery Notes | The phage was discovered under an apple tree, in Somerville, TN, during the beginning of August. The soil was extracted from the base of the tree closest to the trunk. The soil was cool and damp. |
| Naming Notes | The phage was found under an apple tree in an apple orchard. The name AngryOrchard stems from the thought of the orchard having angry phages, from the number of plagues. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 23, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2173 |
| Genome length (bp) | 46597 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGTGAT |
| GC Content | 58.8% |
| Sequencing Notes | There was a polymorphism in the reads for this genome. About 40% of the reads at position 21686 had a "G" in them, instead of the final reported base of "A". |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The AngryOrchard plagues started as cloudy, small plagues, but after a second spot test they became clear and remained small. The plagues appeared within 18-24 hours. |
| Number of Genes | 65 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KY549153 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
22 |
| Pitt Freezer Box Grid# |
H5 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
