Rhodococcus phage Naiad
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Detailed Information for Phage Naiad
Discovery Information
Isolation HostRhodococcus erythropolis RIA 643
Found ByBradley Koch and Brittany Lubich
Year Found2014
Location FoundHudson, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates44.979117 N, 92.759906 W Map
Discovery Notessoil from east bank of St Croix River, near kayak launch site, next to small stream running from the river to a walking path
We think Naiad is a clear plaque mutant of StCroix. They have 2 nt differences and one is in the repressor gene, which change an Arg to a Leu near the HTH region
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 21, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3861
Genome length (bp)46619
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGTGAT
End Determination NotesClear read buildups in Illumina sequencing data.
GC Content58.6%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Noteslarge, clear plaques, 1-2 mm diameter, appearing at 24 hr. Separated from turbid plaques in same sample (turbid plaques isolated as StCroix)
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF324901
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.16 x 10^10
Date of SEA Lysate Titering Oct 30, 2014
Pitt Freezer Box# 12
Pitt Freezer Box Grid# H2
Available Files
EM PictureDownload
GenBank File for PhameratorDownload