Rhodococcus phage Hiro
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Detailed Information for Phage Hiro
Discovery Information
Isolation HostRhodococcus erythropolis RIA 643
Found ByBrittany Sisson
Year Found2014
Location FoundBoston, MA United States
Finding InstitutionFlorida Gulf Coast University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.347053 N, 71.134128 W Map
Discovery NotesHiro was isolated from a sample collected in the Boston area (90 Brainerd Road, Boston, MA 02134, USA). The sample was gathered and shipped to me by FGCU alumni, Michaela Smith.
Naming NotesHiro got its name from my favorite character on the television series, Heroes. The meaning of the name literally translates to "prosperous." I named my phage accordingly because although I failed nearly 30 times before finding Hiro, I ended up with one of the most sturdy lytic phage found within the class.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 5, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2220
Genome length (bp)46854
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGTGAT
End Determination NotesRead buildups in Illumina sequencing data.
GC Content58.7%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesCurrently, Hiro has a titer of 1.0 x 10^8 pfu/mL and a diameter of 2.5mm.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF324898
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 1.0 x 10^8 pfu/mL
Date of SEA Lysate Titering Oct 31, 2014
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload