Rhodococcus phage Espica
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Detailed Information for Phage Espica
Discovery Information
Isolation HostRhodococcus erythropolis RIA 643
Found ByLeah Roach
Year Found2014
Location FoundFort Myers, FL USA
Finding InstitutionFlorida Gulf Coast University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates26.419272 N, 81.807501 W Map
Discovery NotesThe phage was isolated from a feces sample pulled from a manure pile at a horse far. There were six horses and a mule contributing to this pile, one of the horses was ill. It is unclear whether the sample comes from the sick or the healthy horses.The sample was initially pulled by Cassandra Ellis.
Naming NotesI chose the name in part for the binary star system Spica and then added the E to the front because I like the way it sounds.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 5, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2649
Genome length (bp)46537
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGTGAT
End Determination NotesBuildups of reads in Illumina shotgun sequencing.
GC Content58.6%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionAlliance University (formerly Nyack College)
Annotation StatusIn GenBank
Plaque NotesPlaque diameter was consistently approximately 1mm.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK524487
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 124
Pitt Freezer Box Grid# C5
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload