Detailed Information for Phage Rhodalysa |
Discovery Information |
Isolation Host | Rhodococcus erythropolis RIA 643 |
Found By | Juliet Downie |
Year Found | 2014 |
Location Found | Williamsburg, VA USA |
Finding Institution | College of William & Mary |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 37.282925 N, 76.664688 W Map |
Discovery Notes | Soil sample was isolated from compost soil. It underwent two rounds of purification before beginning web lysis process.
Phage measurements: Capsid diameter = 60-70 nm Tail length = 150-200 nm |
Naming Notes | The bacterial host was Rhodococcus globerulus; the phage lyses the bacteria, so it is a "lyser" or "lysa" of "Rhoda", hence "Rhoda""lyser", or "Rhodalysa". I also liked how this name can be pronounced "rhoda-LEE-sah", so it rhymes with Mona Lisa. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 28, 2015 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 2782 |
Genome length (bp) | 46596 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGCCGTGAT |
GC Content | 58.5% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | CA |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Plaques appeared small, clear, and circular. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KT375356 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
6.10 x 10^8 PFU/mL |
Date of SEA Lysate Titering |
Nov 4, 2014 |
Pitt Freezer Box# |
9 |
Pitt Freezer Box Grid# |
G12 |
Available Files |
Plaque Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |