| Detailed Information for Phage AppleCloud |
| Discovery Information |
| Isolation Host | Rhodococcus erythropolis RIA 643 |
| Found By | Kristin Graham and McKenzi Lorrig |
| Year Found | 2014 |
| Location Found | Hudson, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 44.984444 N, 92.754444 W Map |
| Discovery Notes | from same soil sample as Alatin, flower garden soil, semi-dry and loose |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 488 |
| Genome length (bp) | 46389 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGCCGTGAT |
| End Determination Notes | Buildup of reads in Illumina sequencing data. |
| GC Content | 58.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | large, clear plaques, no halo, 2 mm diameter, appearing after 24 hr culture at 30oC |
| Morphotype | Siphoviridae |
| Number of Genes | 64 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF324903 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.2 x 10^9 |
| Date of SEA Lysate Titering |
Oct 20, 2014 |
| Pitt Freezer Box# |
12 |
| Pitt Freezer Box Grid# |
G9 |
| Available Files |
| GenBank File for Phamerator | Download |