The Actinobacteriophage Database

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Detailed Information for Phage Atuin
Discovery Information
Isolation Host	Arthrobacter globiformis B-2979
Found By	Lilianna Rolands
Year Found	2019
Location Found	River Falls, WI United States
Finding Institution	University of Wisconsin-River Falls
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	28°C
GPS Coordinates	44.853056 N, 92.627778 W Map
Discovery Notes	The soil was collected from a small forested area behind Stratton Hall on the UW-River Falls campus. It was cloudy, a little chilly, and had been raining.The soil was damp and packed.
Naming Notes	Named after the world-carrying space turtle in the Discworld books by Sir Terry Pratchett.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 27, 2020
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Approximate Shotgun Coverage	575
Genome length (bp)	194200
Character of genome ends	Direct Terminal Repeat
Direct Terminal Repeat Length	17312 bp
End Determination Notes	At first, we thought Cluster FC phages had no defined ends and were circularly permuted, but as we sequenced more, it became clear that the genomes had a region of double coverage, which is characteristic of a direct terminal repeat. As with some other phages, the right end of the terminal repeat is well-defined, while the left end is less clear.
GC Content	54.2%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	FC
Subcluster	--
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Atuin Bloom Chilliams DunneganBoMo Ellewin GoldenEssence KSunshine22 LeoJr Mimi Panchaali Patbob Phrampa Racecar ReginaGlobina Rockabye SJReid Talia1610 WaddleDee
Annotating Institution	University of Wisconsin-River Falls
Annotation Status	In GenBank
Plaque Notes	Plaques are small pinpoints, about 1mm in diameter, and are round, and clear. They have no halo and are uniform.
Morphotype	Siphoviridae
Number of Genes	307
Number of tRNAs	29
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Atuin_1 Atuin_2 Atuin_3 Atuin_4 Atuin_5 Atuin_6 Atuin_7 Atuin_8 Atuin_9 Atuin_10 Atuin_11 Atuin_12 Atuin_13 Atuin_14 Atuin_15 Atuin_16 Atuin_17 Atuin_18 Atuin_19 Atuin_20 Atuin_21 Atuin_22 Atuin_23 Atuin_24 Atuin_25 Atuin_26 Atuin_27 Atuin_28 Atuin_29 Atuin_30 Atuin_31 Atuin_32 Atuin_33 Atuin_34 Atuin_35 Atuin_36 Atuin_37 Atuin_38 Atuin_39 Atuin_40 Atuin_41 (tail needle protein) Atuin_42 (minor tail protein) Atuin_43 Atuin_44 Atuin_45 Atuin_46 Atuin_47 Atuin_48 Atuin_49 (DNA binding protein) Atuin_50 Atuin_51 Atuin_52 Atuin_53 Atuin_54 (ADP-ribosyltransferase) Atuin_55 Atuin_56 Atuin_57 Atuin_58 (endolysin) Atuin_59 (DprA-like DNA processing chain A) Atuin_60 Atuin_61 Atuin_62 (phosphoribosyl transferase) Atuin_63 (nicotinate ribosyltransferase) Atuin_64 Atuin_65 (metallophosphoesterase) Atuin_66 (helix-turn-helix DNA binding domain) Atuin_67 Atuin_68 Atuin_69 Atuin_70 (portal protein) Atuin_71 (DNA helicase) Atuin_72 Atuin_73 Atuin_74 Atuin_75 (capsid maturation protease) Atuin_76 (major capsid hexamer protein) Atuin_77 Atuin_78 Atuin_79 (head-to-tail adaptor) Atuin_80 Atuin_81 Atuin_82 Atuin_83 (tail assembly chaperone) Atuin_84 Atuin_85 (tape measure protein) Atuin_86 (minor tail protein) Atuin_87 (minor tail protein) Atuin_88 (minor tail protein) Atuin_89 (minor tail protein) Atuin_90 Atuin_91 Atuin_92 Atuin_93 (DNA polymerase I) Atuin_94 (RuvC-like resolvase) Atuin_95 (DNA helicase) Atuin_96 (ThyX-like thymidylate synthase) Atuin_97 (Cas4-family exonuclease) Atuin_98 (RecA-like DNA recombinase) Atuin_99 (ssDNA binding protein) Atuin_100 (MazG-like nucleotide pyrophosphohydrolase) Atuin_101 Atuin_102 (NrdH-like glutaredoxin) Atuin_103 (ribonucleotide reductase) Atuin_104 (ribonucleotide reductase) Atuin_105 (ribonucleotide reductase) Atuin_106 Atuin_107 (phosphatase) Atuin_108 — tRNA (tRNA-Trp(cca)) Atuin_109 — tRNA (tRNA-Pro(tgg)) Atuin_110 — tRNA (tRNA-Gly(tcc)) Atuin_111 (Ro-like RNA binding protein) Atuin_112 — tRNA (tRNA-Thr(tgt)) Atuin_113 — tRNA (tRNA-Phe(gaa)) Atuin_114 — tRNA (tRNA-Gln(ttg)) Atuin_115 Atuin_116 — tRNA (tRNA-Gln(ctg)) Atuin_117 — tRNA (tRNA-Ile(gat)) Atuin_118 Atuin_119 — tRNA (tRNA-Glu(ttc)) Atuin_120 — tRNA (tRNA-Glu(ctc)) Atuin_121 — tRNA (tRNA-Ser(tga)) Atuin_122 — tRNA (tRNA-Ser(gga)) Atuin_123 — tRNA (tRNA-Val(tac)) Atuin_124 — tRNA (tRNA-Leu(caa)) Atuin_125 — tRNA (tRNA-Met(cat)) Atuin_126 — tRNA (tRNA-Arg(acg)) Atuin_127 — tRNA (tRNA-Leu(gag)) Atuin_128 Atuin_129 — tRNA (tRNA-Leu(tag)) Atuin_130 — tRNA (tRNA-Arg(tct)) Atuin_131 — tRNA (tRNA-Tyr(gta)) Atuin_132 — tRNA (tRNA-Lys(ctt)) Atuin_133 — tRNA (tRNA-Lys(ttt)) Atuin_134 Atuin_135 Atuin_136 (nuclease) Atuin_137 (endolysin) Atuin_138 (endolysin, protease domain) Atuin_139 Atuin_140 (DnaE-like DNA polymerase III (alpha)) Atuin_141 Atuin_142 — tRNA (tRNA-Met(cat)) Atuin_143 — tRNA (tRNA-Asn(gtt)) Atuin_144 — tRNA (tRNA-Ala(tgc)) Atuin_145 Atuin_146 — tRNA (tRNA-Asp(gtc)) Atuin_147 Atuin_148 Atuin_149 Atuin_150 Atuin_151 Atuin_152 — tRNA (tRNA-His(gtg)) Atuin_153 (UDP-glucose dehydrogenase) Atuin_154 Atuin_155 Atuin_156 Atuin_157 (phosphatase) Atuin_158 — tRNA (tRNA-Thr(ggt)) Atuin_159 — tRNA (tRNA-Cys(gca)) Atuin_160 (HNH endonuclease) Atuin_161 Atuin_162 (Mre11 double-strand break endo/exonuclease) Atuin_163 Atuin_164 Atuin_165 (glycosyltransferase) Atuin_166 Atuin_167 Atuin_168 Atuin_169 (glycosyltransferase) Atuin_170 (glycosyltransferase) Atuin_171 (nucleotidyl transferase) Atuin_172 Atuin_173 (RNA ligase) Atuin_174 Atuin_175 (polynucleotide kinase) Atuin_176 Atuin_177 Atuin_178 (tRNA nucleotidyl transferase) Atuin_179 (RecA-like DNA recombinase) Atuin_180 Atuin_181 Atuin_182 Atuin_183 (nucleotidyl transferase) Atuin_184 (AAA-ATPase) Atuin_185 Atuin_186 (WhiB family transcription factor) Atuin_187 (DNA helicase) Atuin_188 (DNA primase) Atuin_189 Atuin_190 Atuin_191 Atuin_192 (DNA helicase) Atuin_193 Atuin_194 Atuin_195 Atuin_196 Atuin_197 (lipoprotein) Atuin_198 (lipoprotein) Atuin_199 Atuin_200 Atuin_201 Atuin_202 (terminase) Atuin_203 Atuin_204 Atuin_205 Atuin_206 Atuin_207 Atuin_208 Atuin_209 Atuin_210 Atuin_211 Atuin_212 Atuin_213 Atuin_214 Atuin_215 (glycoside hydrolase) Atuin_216 Atuin_217 Atuin_218 Atuin_219 Atuin_220 Atuin_221 Atuin_222 Atuin_223 Atuin_224 Atuin_225 Atuin_226 Atuin_227 Atuin_228 Atuin_229 Atuin_230 Atuin_231 Atuin_232 Atuin_233 Atuin_234 Atuin_235 Atuin_236 Atuin_237 Atuin_238 Atuin_239 Atuin_240 Atuin_241 Atuin_242 Atuin_243 Atuin_244 Atuin_245 Atuin_246 Atuin_247 Atuin_248 Atuin_249 Atuin_250 Atuin_251 Atuin_252 Atuin_253 Atuin_254 Atuin_255 Atuin_256 Atuin_257 Atuin_258 Atuin_259 Atuin_260 Atuin_261 Atuin_262 Atuin_263 Atuin_264 Atuin_265 Atuin_266 Atuin_267 Atuin_268 Atuin_269 Atuin_270 Atuin_271 Atuin_272 Atuin_273 Atuin_274 Atuin_275 Atuin_276 Atuin_277 Atuin_278 Atuin_279 Atuin_280 Atuin_281 Atuin_282 Atuin_283 Atuin_284 Atuin_285 Atuin_286 Atuin_287 Atuin_288 Atuin_289 Atuin_290 Atuin_291 Atuin_292 Atuin_293 Atuin_294 Atuin_295 Atuin_296 Atuin_297 Atuin_298 Atuin_299 Atuin_300 Atuin_301 Atuin_302 Atuin_303 Atuin_304 Atuin_305 Atuin_306 Atuin_307 Atuin_308 Atuin_309 Atuin_310 Atuin_311 Atuin_312 Atuin_313 Atuin_314 Atuin_315 Atuin_316 Atuin_317 Atuin_318 Atuin_319 Atuin_320 Atuin_321 Atuin_322 Atuin_323 Atuin_324 Atuin_325 Atuin_326 Atuin_327 Atuin_328 Atuin_329 Atuin_330 Atuin_331 Atuin_332 Atuin_333 Atuin_334 Atuin_335 Atuin_336
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	OK040794
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	102
Pitt Freezer Box Grid#	E3
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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