| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Lilianna Rolands |
| Year Found | 2019 |
| Location Found | River Falls, WI United States |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.853056 N, 92.627778 W Map |
| Discovery Notes | The soil was collected from a small forested area behind Stratton Hall on the UW-River Falls campus. It was cloudy, a little chilly, and had been raining.The soil was damp and packed. |
| Naming Notes | Named after the world-carrying space turtle in the Discworld books by Sir Terry Pratchett. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 27, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 575 |
| Genome length (bp) | 194200 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 17312 bp |
| End Determination Notes | At first, we thought Cluster FC phages had no defined ends and were circularly permuted, but as we sequenced more, it became clear that the genomes had a region of double coverage, which is characteristic of a direct terminal repeat. As with some other phages, the right end of the terminal repeat is well-defined, while the left end is less clear. |
| GC Content | 54.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | FC |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are small pinpoints, about 1mm in diameter, and are round, and clear. They have no halo and are uniform. |
| Morphotype | Siphoviridae |
| Number of Genes | 307 |
| Number of tRNAs | 29 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OK040794 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
102 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
