Streptomyces phage BRock
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Detailed Information for Phage BRock
Discovery Information
Isolation HostStreptomyces sp. baron 5a
Former namesStreptomyces sp. SFB5A
Found ByAshley Crossman
Year Found2016
Location FoundBridgewater, VA USA
Finding InstitutionJames Madison University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates38.379099 N, 78.965025 W Map
Discovery NotesPhage BRock was isolated from rich compost with Streptomyces sp. SFB5A (Streptomyces nymphaeiformis) as the host. This streptomycete is a polyhydroxybutyrate-degrading bacterium isolated from decaying mulch; its closest relatives by 16S rDNA sequencing analysis are Streptomyces vietnamensis, Streptomyces tanashiensis, Streptomyces litmocidini, and Streptomyces nashvillensis.
Naming NotesBRock stands for "Baron Rocks". This means that Ashley really admires her teacher!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 12, 2016
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage198
Genome length (bp)112523
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length221 bp
GC Content52.3%
Sequencing NotesThis phage genome is submitted to GenBank in the reverse orientation and will not match our files. Note that the Phamerator file has been turned around to fit standard orientation with structural genes in the forward direction.
Fasta file available?Yes: Download fasta file
Characterization
ClusterBS
Subcluster--
Cluster Life CycleUnknown
Lysogeny NotesColonies form within clearing zones, suggesting that the phage is capable of lysogeny.
Other Cluster Members

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Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesClear plaques with somewhat ragged edges, about 0.5-1.0 mm in diameter.
MorphotypeMyoviridae
Number of Genes188
Number of tRNAs29
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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BRock_1
(HNH endonuclease)

BRock_2

BRock_3

BRock_4

BRock_5

BRock_6

BRock_7

BRock_8
(collagen triple helix repeat-containing protein)

BRock_9 — tRNA

BRock_10 — tRNA

BRock_11 — tRNA

BRock_12 — tRNA

BRock_13

BRock_14 — tRNA

BRock_15 — tRNA

BRock_16 — tRNA

BRock_17 — tRNA

BRock_18 — tRNA

BRock_19 — tRNA

BRock_20

BRock_21 — tRNA

BRock_22 — tRNA

BRock_23 — tRNA

BRock_24 — tRNA

BRock_25 — tRNA

BRock_26 — tRNA

BRock_27 — tRNA

BRock_28
(RNA-binding protein)

BRock_29

BRock_30 — tRNA

BRock_31 — tRNA

BRock_32
(collagen triple helix repeat-containing protein)

BRock_33 — tRNA

BRock_34

BRock_35
(stress protein)

BRock_36

BRock_37 — tRNA

BRock_38

BRock_39

BRock_40 — tRNA

BRock_41

BRock_42

BRock_43 — tRNA

BRock_44 — tRNA

BRock_45

BRock_46 — tRNA

BRock_47 — tRNA

BRock_48 — tRNA

BRock_49
(thioredoxin)

BRock_50 — tRNA

BRock_51
(RNA ligase)

BRock_52 — tRNA

BRock_53

BRock_54
(RNA ligase)

BRock_55

BRock_56
(metallo-dependent phosphatase domain protein)

BRock_57
(phosphoribosyl-ATP diphosphatase)

BRock_58

BRock_59

BRock_60

BRock_61

BRock_62
(terminase large subunit)

BRock_63

BRock_64

BRock_65

BRock_66

BRock_67

BRock_68

BRock_69
(portal protein)

BRock_70

BRock_71
(major capsid protein)

BRock_72

BRock_73

BRock_74

BRock_75

BRock_76

BRock_77

BRock_78

BRock_79

BRock_80
(tail sheath protein)

BRock_81
(tail protein)

BRock_82
(baseplate protein)

BRock_83

BRock_84
(endolysin)

BRock_85
(late control D protein)

BRock_86

BRock_87
(late control D protein)

BRock_88
(tail protein)

BRock_89
(baseplate protein)

BRock_90
(baseplate assembly protein)

BRock_91

BRock_92

BRock_93

BRock_94

BRock_95

BRock_96

BRock_97

BRock_98

BRock_99

BRock_100

BRock_101

BRock_102
(tape measure protein)

BRock_103

BRock_104

BRock_105
(DNA replication protein)

BRock_106

BRock_107

BRock_108
(nucleotidyltransferase)

BRock_109
(polynucleotide kinase)

BRock_110

BRock_111

BRock_112

BRock_113

BRock_114

BRock_115
(DNA helicase)

BRock_116
(DNA primase)

BRock_117
(DNA helicase)

BRock_118

BRock_119

BRock_120

BRock_121

BRock_122

BRock_123

BRock_124

BRock_125

BRock_126

BRock_127

BRock_128

BRock_129

BRock_130

BRock_131

BRock_132

BRock_133
(DNA polymerase I)

BRock_134

BRock_135

BRock_136
(ribonucleotide-diphosphate reductase)

BRock_137

BRock_138

BRock_139
(tail fiber protein)

BRock_140

BRock_141
(RNA ligase)

BRock_142

BRock_143

BRock_144

BRock_145

BRock_146

BRock_147

BRock_148

BRock_149

BRock_150
(transcriptional regulator)

BRock_151

BRock_152

BRock_153

BRock_154
(thymidylate synthase)

BRock_155

BRock_156

BRock_157

BRock_158

BRock_159

BRock_160

BRock_161

BRock_162
(iSH4-type transposase domain protein)

BRock_163
(ssDNA binding protein)

BRock_164

BRock_165

BRock_166

BRock_167

BRock_168

BRock_169

BRock_170

BRock_171

BRock_172

BRock_173

BRock_174
(glycoside hydrolase)

BRock_175
(transglycosylase)

BRock_176

BRock_177

BRock_178
(endolysin)

BRock_179

BRock_180

BRock_181

BRock_182

BRock_183

BRock_184

BRock_185

BRock_186

BRock_187

BRock_188

BRock_189

BRock_190

BRock_191

BRock_192

BRock_193

BRock_194

BRock_195

BRock_196

BRock_197

BRock_198

BRock_199

BRock_200

BRock_201

BRock_202

BRock_203

BRock_204

BRock_205

BRock_206

BRock_207

BRock_208

BRock_209

BRock_210

BRock_211

BRock_212

BRock_213

BRock_214

BRock_215

BRock_216

BRock_217

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX925554
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
EM PictureDownload