| Detailed Information for Phage BRock |
| Discovery Information |
| Isolation Host | Streptomyces sp. baron 5a |
| Former names | Streptomyces sp. SFB5A |
| Found By | Ashley Crossman |
| Year Found | 2016 |
| Location Found | Bridgewater, VA USA |
| Finding Institution | James Madison University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 38.379099 N, 78.965025 W Map |
| Discovery Notes | Phage BRock was isolated from rich compost with Streptomyces sp. SFB5A (Streptomyces nymphaeiformis) as the host. This streptomycete is a polyhydroxybutyrate-degrading bacterium isolated from decaying mulch; its closest relatives by 16S rDNA sequencing analysis are Streptomyces vietnamensis, Streptomyces tanashiensis, Streptomyces litmocidini, and Streptomyces nashvillensis. |
| Naming Notes | BRock stands for "Baron Rocks". This means that Ashley really admires her teacher! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 12, 2016 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 198 |
| Genome length (bp) | 112523 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 221 bp |
| GC Content | 52.3% |
| Sequencing Notes | This phage genome is submitted to GenBank in the reverse orientation and will not match our files. Note that the Phamerator file has been turned around to fit standard orientation with structural genes in the forward direction. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BS |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | Colonies form within clearing zones, suggesting that the phage is capable of lysogeny. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques with somewhat ragged edges, about 0.5-1.0 mm in diameter. |
| Morphotype | Myoviridae |
| Number of Genes | 188 |
| Number of tRNAs | 29 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX925554 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| EM Picture | Download |