| Detailed Information for Phage BarnCat |
| Discovery Information |
| Isolation Host | Microbacterium paraoxydans NRRL B-14843 |
| Found By | Tahmara Hendrickson |
| Year Found | 2021 |
| Location Found | Viroqua, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 43.494722 N, 90.863889 W Map |
| Discovery Notes | Soil Info: mix of wet/dry soil, near my dog's potty place. No roots, minimal grass.
Weather conditions: cool, misty/slightly wet, low humidity.
Animals nearby: my dog and many cats. |
| Naming Notes | The origin of the name is that he was my old barn cat that I rescued from under the lawnmower as a kitten at the vet clinic I used to work at. He was my favorite cat for a long time. He was hit by a car a little over a year after I brought him home. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 15, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 638 |
| Genome length (bp) | 61511 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1507 bp |
| GC Content | 58.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | GB |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | La Sierra University |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques are relatively large compared to others in my class. As the rounds of purification and dilutions progressed, they became more uniform in size, but the photo I am using has multiple variations in size. They remained clear the whole time, and sometimes had a halo, sometimes did not. |
| Morphotype | Siphoviridae |
| Number of Genes | 125 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978804 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
140 |
| Pitt Freezer Box Grid# |
B7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
