Microbacterium phage Cassita
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Detailed Information for Phage Cassita
Discovery Information
Isolation HostMicrobacterium paraoxydans NRRL B-14843
Found ByNicole Grant
Year Found2021
Location FoundHudson, WI United States
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature32°C
GPS Coordinates44.984533 N, 92.7545 W Map
Discovery NotesDate collected: 9/8/21 @ 7:00AM

Depth of Collection: 5 cm

Weather Conditions: 63⁰F, dry, sunny, slight wind

Sample Site Description: compost pile in alleyway, semi-moist, partially degraded leaves and grass

-No live plants nearby
-Squirrels, chipmunks, spiders, earthworms, various insects observed here
-From Karen Klyczek's soil sample
Naming NotesMy favorite horse from my work and volunteer barn, Main Stay Therapeutic Farm, was named Cassa. She was recently put down because of complications with her hooves, where her condition was not improving. So the best option for her was to be laid to rest to be free of any further pain and discomfort. This phage is my tribute to her, as a little version of her, with the suffix "-ita" meaning "little" in Spanish.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 26, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1220
Genome length (bp)61868
Character of genome endsUnknown
Direct Terminal Repeat Length1649 bp
GC Content57.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterGB
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusIn GenBank
Plaque NotesSize: about 1mm

Appearance: clear center, medium-sized turbid halo

-Relatively uniform throughout; some smaller size
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Cassita_1

Cassita_2

Cassita_3

Cassita_4

Cassita_5

Cassita_6

Cassita_7

Cassita_8

Cassita_9

Cassita_10

Cassita_11

Cassita_12

Cassita_13

Cassita_14

Cassita_15

Cassita_16
(protease)

Cassita_17

Cassita_18

Cassita_19

Cassita_20

Cassita_21

Cassita_22
(DNA binding protein)

Cassita_23
(terminase)

Cassita_24
(portal protein)

Cassita_25
(capsid maturation protease)

Cassita_26

Cassita_27
(major capsid protein)

Cassita_28
(G-I-Y Y-I-G endonuclease)

Cassita_29
(minor tail protein)

Cassita_30
(head fiber protein)

Cassita_31

Cassita_32
(glycoside hydrolase)

Cassita_33
(hydrolase)

Cassita_34

Cassita_35
(head-to-tail stopper)

Cassita_36

Cassita_37
(tail terminator)

Cassita_38

Cassita_39

Cassita_40
(major tail protein)

Cassita_41
(tail assembly chaperone)

Cassita_42
(tail assembly chaperone)

Cassita_43
(tape measure protein)

Cassita_44
(minor tail protein)

Cassita_45
(minor tail protein)

Cassita_46
(minor tail protein)

Cassita_47

Cassita_48

Cassita_49
(endolysin)

Cassita_50
(exonuclease)

Cassita_51

Cassita_52

Cassita_53

Cassita_54
(NrdH-like glutaredoxin)

Cassita_55

Cassita_56

Cassita_57

Cassita_58

Cassita_59

Cassita_60
(RuvC-like resolvase)

Cassita_61

Cassita_62

Cassita_63

Cassita_64

Cassita_65
(DNA polymerase I)

Cassita_66

Cassita_67
(acetyltransferase)

Cassita_68
(helix-turn-helix DNA binding domain)

Cassita_69

Cassita_70

Cassita_72
(ssDNA binding protein)

Cassita_73

Cassita_74

Cassita_75
(exonuclease)

Cassita_76

Cassita_77

Cassita_78

Cassita_79
(ssDNA binding protein, ERF family)

Cassita_80

Cassita_81
(DnaQ-like (DNA polymerase III subunit))

Cassita_82

Cassita_83

Cassita_84
(WhiB family transcription factor)

Cassita_85

Cassita_86

Cassita_87

Cassita_88
(HNH endonuclease)

Cassita_89
(DNA helicase)

Cassita_90
(DNA binding protein)

Cassita_91

Cassita_92

Cassita_93

Cassita_94

Cassita_95

Cassita_96

Cassita_97

Cassita_98

Cassita_99

Cassita_100

Cassita_101

Cassita_102

Cassita_103

Cassita_104

Cassita_105

Cassita_106

Cassita_107

Cassita_108

Cassita_109

Cassita_110

Cassita_111

Cassita_112

Cassita_113

Cassita_114

Cassita_115

Cassita_116

Cassita_117

Cassita_118

Cassita_119

Cassita_120

Cassita_121

Cassita_122

Cassita_123

Cassita_124

Cassita_125

Cassita_126

Cassita_127

Cassita_128

Cassita_129

Cassita_130

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON526969
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 140
Pitt Freezer Box Grid# B9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload