Microbacterium phage Lifes
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Detailed Information for Phage Lifes
Discovery Information
Isolation HostMicrobacterium paraoxydans NRRL B-14843
Former namesLiFES
Found ByOlivia Gronholz
Year Found2018
Location FoundGreen Isle, MN USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates44.679121 N, 94.012799 W Map
Discovery NotesSoil sample was very dry and clumpy. The dirt was loose.
Soil was taken from a newly planted tree; this is where multiple trees have died.
The soil was taken from a depth of 80 mm.
The temperature was 70 degrees F and it was sunny, not humid, and there was a breeze.
Naming NotesThe name is inspired by names in my life. There are some things with names that start with L,F,E, and S in my life. I decided to make it an acronym, becoming LiFES.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 27, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage392
Genome length (bp)59253
Character of genome endsDirect Terminal Repeat
Overhang Length1350 bases
GC Content58.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterGB
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusIn GenBank
Plaque NotesThe plaques were all very clear. They had no halos and they were all uniform in size.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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(protease)

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(terminase)

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(portal protein)

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(capsid maturation protease)

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(major capsid protein)

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(tailspike protein)

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(hydrolase)

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(tail terminator)

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(major tail protein)

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(tail assembly chaperone)

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(tail assembly chaperone)

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(tape measure protein)

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(minor tail protein)

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(minor tail protein)

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(minor tail protein)

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(endolysin)

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(exonuclease)

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(NrdH-like glutaredoxin)

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(RuvC-like resolvase)

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(nucleotidyl transferase)

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(DNA polymerase I)

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(acetyltransferase)

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(helix-turn-helix DNA binding domain protein)

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(DNA primase)

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(ssDNA-binding protein)

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(exonuclease)

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(ERF family ssDNA binding protein)

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(exonuclease)

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(HNH endonuclease)

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(WhiB family transcription factor)

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(DNA helicase)

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Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT818426
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 87
Pitt Freezer Box Grid# H6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload