| Detailed Information for Phage Belenaria |
| Discovery Information |
| Isolation Host | Rhodococcus erythropolis RIA 643 |
| Found By | Belen Rodriguez |
| Year Found | 2014 |
| Location Found | Boston, MA USA |
| Finding Institution | Florida Gulf Coast University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.347015 N, 71.134265 W Map |
| Discovery Notes | The sample was collected by a Florida Gulf Coast University alumni, Michaela Smith, in Boston, Massachusetts. The student collected several soil samples from Boston and sent them to Florida Gulf Coast University. |
| Naming Notes | The name "Belenaria" is derived from the discoverer's name. I added "aria" at the end so it could sound more like an actual virus. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3275 |
| Genome length (bp) | 46538 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGTGAT |
| End Determination Notes | Buildups of reads from Illumina sequencing. |
| GC Content | 58.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Alliance University (formerly Nyack College) |
| Annotation Status | In GenBank |
| Plaque Notes | The Boston sample produces very lytic plaques. The average diameter is approximately a little bit less than one millimeter long. The titer for the medium titer lysate is 6.14 x 10^5 pfu/mL. |
| Morphotype | Siphoviridae |
| Number of Genes | 68 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524495 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
124 |
| Pitt Freezer Box Grid# |
C1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
