The Actinobacteriophage Database

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Detailed Information for Phage BigMama
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Former names	Meatball
Found By	Dana Figel
Year Found	2012
Location Found	Washington, DC, DC USA
Finding Institution	St. Andrew's Episcopal School
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	Not entered
GPS Coordinates	38.930852 N, 77.103008 W Map
Discovery Notes	3114 51st Pl. Washington DC 20016 dirt from lawn in backyard
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Oct 15, 2013
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Ion Torrent
Genome length (bp)	64592
Character of genome ends	Unknown
GC Content	59.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	D
Subcluster	D1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Adjutor (D1) BigMama (D1) Butterscotch (D1) Chill (D1) Delton (D1) Erk16 (D1) Giuseppe (D1) Gumball (D1) Helpful (D1) KandZ (D1) Mopey (D1) Nova (D1) PBI1 (D1) Penelope2018 (D1) PLot (D1) Prager (D1) SirHarley (D1) Thoth (D1) Troll4 (D1) Visconti (D1) WaldoWhy (D1) Hawkeye (D2)
Annotating Institution	St. Andrew's Episcopal School
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	87
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View BigMama_1 BigMama_2 BigMama_3 BigMama_4 (capsid accessory protein) BigMama_5 BigMama_6 (VIP2-like toxin) BigMama_7 (terminase) BigMama_8 (portal protein) BigMama_9 BigMama_10 BigMama_11 BigMama_12 BigMama_13 (minor capsid protein) BigMama_14 (major capsid protein) BigMama_15 BigMama_16 BigMama_17 BigMama_18 BigMama_19 BigMama_20 BigMama_21 BigMama_22 (major tail protein) BigMama_23 (tail assembly chaperone) BigMama_24 (tail assembly chaperone) BigMama_25 (tape measure protein) BigMama_26 BigMama_27 (minor tail protein) BigMama_28 (minor tail protein) BigMama_29 (D-Ala-D-Ala carboxypeptidase) BigMama_30 (minor tail protein) BigMama_31 BigMama_32 BigMama_33 (lysin A) BigMama_34 BigMama_35 BigMama_36 BigMama_37 BigMama_38 (lysin B) BigMama_39 BigMama_40 BigMama_41 BigMama_42 BigMama_43 BigMama_44 BigMama_45 BigMama_46 BigMama_47 BigMama_48 BigMama_49 BigMama_50 BigMama_51 (DNA helicase) BigMama_52 BigMama_53 BigMama_54 BigMama_55 (exonuclease) BigMama_56 BigMama_57 (DNA recombinase) BigMama_58 BigMama_59 BigMama_60 (oxidoreductase) BigMama_61 (hydrolase) BigMama_62 (methyltransferase) BigMama_63 BigMama_64 BigMama_65 (DNA polymerase III alpha) BigMama_66 BigMama_67 (ThyX-like thymidylate synthase) BigMama_68 (resolvase) BigMama_69 BigMama_70 BigMama_71 BigMama_72 BigMama_73 BigMama_74 BigMama_75 BigMama_76 BigMama_77 BigMama_78 BigMama_79 BigMama_80 BigMama_81 BigMama_82 BigMama_83 BigMama_84 BigMama_85 BigMama_86 BigMama_87 (DNA primase/polymerase)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MH025888
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	3
Pitt Freezer Box Grid#	F12
Available Files
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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