| Detailed Information for Phage Blueeyedbeauty |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Cody Luttrell |
| Year Found | 2016 |
| Location Found | Denton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.211081 N, 97.146375 W Map |
| Discovery Notes | The date that I gathered my soil was September 12, 2016 at 11:21 AM. The soil the day I gathered it was light brown, moist, and grainy. The weather outside at the time of gathering was sunny at 33.56 C. |
| Naming Notes | I am interested in Optometry and I have blue eyes, therefore naming it Blueeyedbeauty |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 14, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 594 |
| Genome length (bp) | 130473 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 788 bp |
| GC Content | 47.9% |
| Sequencing Notes | Genome ends were hard to define. There was approximately a 5945 bp region of 2x coverage but minimal buildup of reads at both the start and end of these positions.
UPDATED:
Based on similarity to Annadreamy, we reduced the probable size of the terminal repeat to 788 bp. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BK |
| Subcluster | BK1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | The average plaque diameter of my phage was 1.5 mm. The area of my plaque was 1.767 mm^2 |
| Number of Genes | 240 |
| Number of tRNAs | 36 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH536814 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8x10^10 pfu/mL |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
D5 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
