Streptomyces phage Forrest
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Detailed Information for Phage Forrest
Discovery Information
Isolation HostStreptomyces viridochromogenes DSM40736
Found ByVu Mather and Amy Chen
Year Found2015
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature24°C
GPS Coordinates38.646667 N, 90.310833 W Map
Discovery NotesFound in the Elizabeth Danforth Butterfly Garden near some milkweed plants. The soil was loose and dry and sample depth was 4 cm.
Naming NotesWe found it in a forest/butterfly garden and we love Forrest Gump..... so why not?
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 29, 2020
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage93
Genome length (bp)128513
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length766 bp
GC Content47.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBK
SubclusterBK1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusBeing Annotated (Expected completion by 3/29/2021)
Plaque NotesAfter four rounds of purification, our plaques consolidated in morphology. They were all small in size, about 1 mm in diameter. Each plaque was also completely clear and had very distinct borders.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5.0x10^10 pfu/mL
Date of SEA Lysate Titering Oct 8, 2015
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload