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Streptomyces phage Forrest
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Detailed Information for Phage Forrest
Discovery Information
Isolation HostStreptomyces viridochromogenes DSM40736
Found ByVu Mather and Amy Chen
Year Found2015
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature24°C
GPS Coordinates38.646667 N, 90.310833 W Map
Discovery NotesFound in the Elizabeth Danforth Butterfly Garden near some milkweed plants. The soil was loose and dry and sample depth was 4 cm.
Naming NotesWe found it in a forest/butterfly garden and we love Forrest Gump..... so why not?
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 29, 2020
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage93
Genome length (bp)128513
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length766 bp
GC Content47.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBK
SubclusterBK1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusIn GenBank
Plaque NotesAfter four rounds of purification, our plaques consolidated in morphology. They were all small in size, about 1 mm in diameter. Each plaque was also completely clear and had very distinct borders.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Forrest_1

Forrest_2

Forrest_3

Forrest_4

Forrest_5

Forrest_6

Forrest_7
(DNA methylase)

Forrest_8
(HNH endonuclease)

Forrest_9

Forrest_10
(ThyX-like thymidylate synthase)

Forrest_11

Forrest_12

Forrest_13

Forrest_14

Forrest_15

Forrest_16
(HNH endonuclease)

Forrest_21

Forrest_22
(glycosyltransferase)

Forrest_23
(glycosyltransferase)

Forrest_24
(ribonucleotide reductase)

Forrest_25
(endolysin)

Forrest_26

Forrest_27

Forrest_28
(portal protein)

Forrest_29

Forrest_30
(capsid maturation protease)

Forrest_31
(major capsid protein)

Forrest_32

Forrest_33
(head-to-tail adaptor)

Forrest_34
(head-to-tail stopper)

Forrest_35

Forrest_36

Forrest_37
(major tail protein)

Forrest_38
(tail assembly chaperone)

Forrest_39
(tail assembly chaperone)

Forrest_40
(tape measure protein)

Forrest_41
(minor tail protein)

Forrest_42
(minor tail protein)

Forrest_43

Forrest_44
(minor tail protein)

Forrest_45
(minor tail protein)

Forrest_46

Forrest_47

Forrest_48

Forrest_49
(HNH endonuclease)

Forrest_50
(HNH endonuclease)

Forrest_51
(metallophosphoesterase)

Forrest_52

Forrest_53
(MerR-like helix-turn-helix DNA binding domain protein)

Forrest_54

Forrest_55

Forrest_56

Forrest_57
(DnaB-like dsDNA helicase)

Forrest_58

Forrest_59
(DNA primase)

Forrest_60
(ssDNA binding protein)

Forrest_61
(HNH endonuclease)

Forrest_62
(DnaE-like DNA polymerase III alpha)

Forrest_63

Forrest_64
(MazG-like nucleotide pyrophosphohydrolase)

Forrest_65

Forrest_66
(RecA-like DNA recombinase)

Forrest_67

Forrest_68

Forrest_69
(PnuC-like nicotinamide riboside transporter)

Forrest_70

Forrest_71

Forrest_72

Forrest_73
(HNH endonuclease)

Forrest_74
(Cas4 family exonuclease)

Forrest_75

Forrest_76
(RuvC-like resolvase)

Forrest_77

Forrest_78
(DprA-like DNA processing chain A)

Forrest_79
(helix-turn-helix DNA binding domain protein)

Forrest_80
(glycosyltransferase)

Forrest_81
(glycosyltransferase)

Forrest_82
(nucleotidyl transferase)

Forrest_83

Forrest_84
(DnaQ-like DNA polymerase III subunit)

Forrest_85
(deoxynucleoside monophosphate kinase)

Forrest_86
(5' nucleotidase)

Forrest_87

Forrest_88

Forrest_89

Forrest_90
(helix-turn-helix DNA binding domain protein)

Forrest_91
(HNH endonuclease)

Forrest_92
(terminase)

Forrest_93

Forrest_94

Forrest_97
(HNH endonuclease)

Forrest_98

Forrest_101

Forrest_104

Forrest_105

Forrest_106
(endonuclease VII)

Forrest_108

Forrest_109

Forrest_110

Forrest_111
(Ro-like RNA binding protein)

Forrest_112

Forrest_113

Forrest_115

Forrest_116
(DnaQ-like DNA polymerase III subunit)

Forrest_117

Forrest_118

Forrest_120

Forrest_122

Forrest_124
(HNH endonuclease)

Forrest_127

Forrest_128

Forrest_129

Forrest_130

Forrest_131

Forrest_134
(LAGLIDADG endonuclease)

Forrest_138

Forrest_139

Forrest_141

Forrest_143

Forrest_144

Forrest_145

Forrest_147

Forrest_148

Forrest_149

Forrest_150
(HNH endonuclease)

Forrest_152

Forrest_153
(RNA ligase)

Forrest_154

Forrest_155
(polynucleotide kinase)

Forrest_156

Forrest_157

Forrest_159

Forrest_160

Forrest_161

Forrest_162

Forrest_165

Forrest_166

Forrest_167

Forrest_168

Forrest_169

Forrest_170

Forrest_171
(HNH endonuclease)

Forrest_172

Forrest_173

Forrest_174
(metallophosphoesterase)

Forrest_175

Forrest_176
(HNH endonuclease)

Forrest_178

Forrest_179
(WhiB family transcription factor)

Forrest_180

Forrest_181

Forrest_182

Forrest_184

Forrest_185

Forrest_186

Forrest_188
(LAGLIDADG endonuclease)

Forrest_191

Forrest_193

Forrest_195

Forrest_197

Forrest_198

Forrest_199

Forrest_200
(HNH endonuclease)

Forrest_201

Forrest_202

Forrest_203

Forrest_204
(thioredoxin)

Forrest_205

Forrest_206

Forrest_207

Forrest_208

Forrest_209
(RNA ligase)

Forrest_210
(HNH endonuclease)

Forrest_211

Forrest_212

Forrest_213

Forrest_214

Forrest_215

Forrest_216

Forrest_217

Forrest_218

Forrest_219
(Lsr2-like DNA bridging protein)

Forrest_220

Forrest_221

Forrest_222

Forrest_223

Forrest_224

Forrest_225

Forrest_226

Forrest_227
(HNH endonuclease)

Forrest_228

Forrest_229

Forrest_230

Forrest_231
(peptidase)

Forrest_232

Forrest_233

Forrest_234

Forrest_235

Forrest_236

Forrest_237
(HNH endonuclease)

Forrest_238
(metallophosphoesterase)

Forrest_239
(SprT-like protease)

Forrest_240

Forrest_241

Forrest_242

Forrest_243

Forrest_244

Forrest_245

Forrest_246

Forrest_248

Forrest_249

Forrest_250

Forrest_251

Forrest_252

Forrest_253

Forrest_254
(HNH endonuclease)

Forrest_255

Forrest_256

Forrest_257

Forrest_258

Forrest_259

Forrest_260

Forrest_261

Forrest_262

Forrest_263

Forrest_264

Forrest_265

Forrest_266

Forrest_267

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ820093
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5.0x10^10 pfu/mL
Date of SEA Lysate Titering Oct 8, 2015
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload