Streptomyces phage Circinus
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Detailed Information for Phage Circinus
Discovery Information
Isolation HostStreptomyces griseofuscus ATCC 23916
Found ByAmy Kwan, Caelan Miller, & Brandon Tang
Year Found2017
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates38.6032 N, 90.2616 W Map
Discovery NotesSpecimen was discovered near a tree by the Seed Sprout Spoon, located in St. Louis, MO.
Naming NotesThe name is inspired by the constellation Circinus and the plaque's stellar shape. Circinus means "the compass" from its translation from Latin. All of our previous samples proved to be difficult in cultivating phage growth until this sample gave us a sense of direction through its successful plaque growth.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 12, 2018
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage86
Genome length (bp)126383
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length2056 bp
GC Content52.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBK
SubclusterBK2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusIn GenBank
Plaque NotesStellar in shape, clear around edges and within plaque, preferentially medium-sized
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Circinus_1

Circinus_2

Circinus_3

Circinus_4

Circinus_5

Circinus_6

Circinus_7

Circinus_8

Circinus_9
(DNA methylase)

Circinus_10
(thymidylate synthase)

Circinus_11

Circinus_15

Circinus_16
(glycosyltransferase)

Circinus_17
(glycosyltransferase)

Circinus_18
(ribonucleotide reductase)

Circinus_19
(LysM-like endolysin)

Circinus_20

Circinus_21

Circinus_22
(portal protein)

Circinus_23
(membrane protein)

Circinus_24
(capsid maturation protease)

Circinus_25
(major capsid protein)

Circinus_26

Circinus_27

Circinus_28

Circinus_29
(head-to-tail adaptor)

Circinus_30

Circinus_31

Circinus_32

Circinus_33
(major tail protein)

Circinus_34
(tail assembly chaperone)

Circinus_35

Circinus_36
(tape measure protein)

Circinus_37
(minor tail protein)

Circinus_38
(minor tail protein)

Circinus_39

Circinus_40
(major tail protein)

Circinus_41

Circinus_42

Circinus_43

Circinus_44

Circinus_45
(HNH endonuclease)

Circinus_46
(membrane protein)

Circinus_47
(phosphoesterase)

Circinus_48
(holin)

Circinus_49
(helix-turn-helix DNA binding domain protein)

Circinus_50

Circinus_51

Circinus_52

Circinus_53
(DNA helicase)

Circinus_54

Circinus_55
(DNA primase)

Circinus_56
(ssDNA binding protein)

Circinus_57
(HNH endonuclease)

Circinus_58
(DnaE-like DNA polymerase III)

Circinus_59

Circinus_60
(deoxyuridine triphosphatase)

Circinus_61

Circinus_62
(RecA-like DNA recombinase)

Circinus_63

Circinus_64

Circinus_65

Circinus_66

Circinus_67
(Cas4 family exonuclease)

Circinus_68

Circinus_69
(RuvC-like resolvase)

Circinus_70
(DprA-like DNA processing chain A)

Circinus_71
(helix-turn-helix DNA binding domain protein)

Circinus_72
(glycosyltransferase)

Circinus_73
(DnaQ-like DNA polymerase III subunit)

Circinus_74
(deoxynucleoside monophosphate kinase)

Circinus_75

Circinus_76

Circinus_77

Circinus_78
(terminase)

Circinus_79

Circinus_80
(peptidase)

Circinus_81

Circinus_84
(HNH endonuclease)

Circinus_85

Circinus_91

Circinus_92

Circinus_93

Circinus_94

Circinus_95

Circinus_96
(Lsr2-like DNA bridging protein)

Circinus_98

Circinus_100

Circinus_101
(phosphoesterase)

Circinus_102
(peptidase)

Circinus_103

Circinus_104

Circinus_110

Circinus_111

Circinus_117

Circinus_120

Circinus_125

Circinus_126

Circinus_127

Circinus_128

Circinus_129

Circinus_130
(membrane protein)

Circinus_131

Circinus_132

Circinus_133
(phosphoesterase)

Circinus_134

Circinus_135

Circinus_136
(WhiB family transcription factor)

Circinus_137

Circinus_138

Circinus_139

Circinus_140
(membrane protein)

Circinus_141

Circinus_142

Circinus_145

Circinus_146

Circinus_147

Circinus_149
(membrane protein)

Circinus_150

Circinus_152
(nuclease)

Circinus_153
(membrane protein)

Circinus_154

Circinus_155
(membrane protein)

Circinus_156

Circinus_157

Circinus_158

Circinus_159

Circinus_160
(thioredoxin)

Circinus_161
(membrane protein)

Circinus_162

Circinus_163

Circinus_164

Circinus_165

Circinus_167
(RNA ligase)

Circinus_168
(peptidase)

Circinus_169

Circinus_170

Circinus_171
(membrane protein)

Circinus_172
(membrane protein)

Circinus_173
(polynucleotide kinase)

Circinus_174
(membrane protein)

Circinus_175

Circinus_176

Circinus_177

Circinus_178
(glycosylase)

Circinus_179

Circinus_180
(membrane protein)

Circinus_181

Circinus_182

Circinus_183

Circinus_184

Circinus_185

Circinus_186

Circinus_187

Circinus_188

Circinus_189

Circinus_190

Circinus_191
(membrane protein)

Circinus_192

Circinus_193

Circinus_194

Circinus_195
(membrane protein)

Circinus_196
(RNA ligase)

Circinus_197

Circinus_198

Circinus_199
(SprT-like protease)

Circinus_200

Circinus_201
(membrane protein)

Circinus_202

Circinus_203

Circinus_204

Circinus_205
(lipoprotein)

Circinus_206
(membrane protein)

Circinus_207

Circinus_208

Circinus_210
(DNA methylase)

Circinus_211

Circinus_212

Circinus_213

Circinus_214

Circinus_215

Circinus_216

Circinus_217

Circinus_218

Circinus_219

Circinus_220

Circinus_221

Circinus_222
(Lsr2-like DNA bridging protein)

Circinus_223
(Lsr2-like DNA bridging protein)

Circinus_224

Circinus_225

Circinus_226

Circinus_227

Circinus_228

Circinus_229

Circinus_230

Circinus_231

Circinus_232

Circinus_233

Circinus_234

Circinus_235

Circinus_236

Circinus_237

Circinus_238

Circinus_239

Circinus_240

Circinus_241

Circinus_242

Circinus_243

Circinus_244

Circinus_245

Circinus_246

Circinus_247

Circinus_248

Circinus_249

Circinus_250

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK620896
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.0*10^9
Pitt Freezer Box# 49
Pitt Freezer Box Grid# C5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload