Streptomyces phage Stigma
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Detailed Information for Phage Stigma
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Found BySydney Gans
Year Found2020
Location FoundDenton, TX United States
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.1227 N, 97.919 W Map
Discovery NotesAbigail Higginbotham Collected soil from (33.1227 N, 97.919 W) at 690 feet elevation in the south west side of the University of North Texas campus. It was 93 degrees at the time of collection. The soil was dry and dark, as it had retained water from rain a few days prior. It was collected under a tree by a parking lot. Abigail collected the top 1 inch of the soil and placed it in a plastic bag. I adopted one of the phages from her soil sample.
Naming NotesI named this phage after a song I always play to fall asleep.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 22, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1246
Genome length (bp)129455
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length734 bp
GC Content47.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBK
SubclusterBK1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesMostly round, some more oval shaped, bullseye plaques about 2mm or less
Has been Phamerated?Yes
Gene List

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Stigma_1

Stigma_2

Stigma_3

Stigma_4

Stigma_5

Stigma_6

Stigma_7
(DNA methyltransferase)

Stigma_8
(ThyX-like thymidylate synthase)

Stigma_9

Stigma_10

Stigma_11

Stigma_12

Stigma_13

Stigma_17

Stigma_18

Stigma_19
(glycosyltransferase)

Stigma_20
(glycosyltransferase)

Stigma_21
(ribonucleotide reductase)

Stigma_22
(endolysin)

Stigma_23

Stigma_24

Stigma_25
(portal protein)

Stigma_26

Stigma_27
(capsid maturation protease)

Stigma_28
(major capsid protein)

Stigma_29

Stigma_30

Stigma_31

Stigma_32
(head-to-tail adaptor)

Stigma_33

Stigma_34

Stigma_35

Stigma_36
(major tail protein)

Stigma_37
(tail assembly chaperone)

Stigma_38
(tail assembly chaperone)

Stigma_39
(tape measure protein)

Stigma_40
(minor tail protein)

Stigma_41
(minor tail protein)

Stigma_42

Stigma_43
(minor tail protein)

Stigma_44
(minor tail protein)

Stigma_45

Stigma_46

Stigma_47

Stigma_48
(HNH endonuclease)

Stigma_49
(metallophosphoesterase)

Stigma_50

Stigma_51
(helix-turn-helix DNA binding domain protein)

Stigma_52

Stigma_53

Stigma_54

Stigma_55
(DnaB-like dsDNA helicase)

Stigma_56

Stigma_57
(DNA primase)

Stigma_58
(ssDNA binding protein)

Stigma_59
(HNH endonuclease)

Stigma_60
(DnaE-like DNA polymerase III alpha)

Stigma_61

Stigma_62
(deoxyuridine triphosphatase)

Stigma_63

Stigma_64
(RecA-like DNA recombinase)

Stigma_65

Stigma_66
(PnuC-like nicotinamide riboside transporter)

Stigma_67

Stigma_68

Stigma_69

Stigma_70
(Cas4 family exonuclease)

Stigma_71

Stigma_72
(RuvC-like resolvase)

Stigma_73

Stigma_74
(DprA-like DNA processing chain A)

Stigma_75
(helix-turn-helix DNA binding domain protein)

Stigma_76
(glycosyltransferase)

Stigma_77
(glycosyltransferase)

Stigma_78
(nucleotidyl transferase)

Stigma_79

Stigma_80
(DnaQ-like DNA polymerase III subunit)

Stigma_81
(deoxynucleoside monophosphate kinase)

Stigma_82
(phosphatase)

Stigma_83

Stigma_84

Stigma_85

Stigma_86

Stigma_87
(terminase)

Stigma_88

Stigma_89

Stigma_92
(HNH endonuclease)

Stigma_95

Stigma_98

Stigma_100

Stigma_101

Stigma_102

Stigma_103
(Ro-like RNA binding protein)

Stigma_104

Stigma_105

Stigma_107

Stigma_108

Stigma_109
(DnaQ-like DNA polymerase III subunit)

Stigma_110

Stigma_111

Stigma_113

Stigma_115

Stigma_116

Stigma_120

Stigma_121

Stigma_122

Stigma_123

Stigma_129

Stigma_132

Stigma_133
(band-7-like membrane protein)

Stigma_134

Stigma_135

Stigma_136

Stigma_137

Stigma_138

Stigma_139

Stigma_140

Stigma_142

Stigma_143

Stigma_144

Stigma_145
(RNA ligase)

Stigma_146

Stigma_147
(polynucleotide kinase)

Stigma_148

Stigma_149

Stigma_151

Stigma_152

Stigma_153

Stigma_154

Stigma_157

Stigma_158

Stigma_159

Stigma_160

Stigma_161

Stigma_162

Stigma_163

Stigma_164

Stigma_165

Stigma_166

Stigma_167
(metallophosphoesterase)

Stigma_168

Stigma_170

Stigma_171
(WhiB family transcription factor)

Stigma_172

Stigma_173

Stigma_174

Stigma_176

Stigma_177

Stigma_178

Stigma_181

Stigma_183

Stigma_184

Stigma_186

Stigma_188

Stigma_189

Stigma_190

Stigma_191

Stigma_192

Stigma_193

Stigma_194

Stigma_195

Stigma_196
(thioredoxin)

Stigma_197

Stigma_198

Stigma_199

Stigma_200
(glycosylase)

Stigma_201

Stigma_202

Stigma_203

Stigma_204

Stigma_205

Stigma_206
(RNA ligase)

Stigma_207

Stigma_208

Stigma_209

Stigma_210

Stigma_211

Stigma_212

Stigma_213

Stigma_214

Stigma_215
(Lsr2-like DNA bridging protein)

Stigma_216

Stigma_217

Stigma_218

Stigma_219

Stigma_220

Stigma_221

Stigma_222

Stigma_223
(HNH endonuclease)

Stigma_224

Stigma_225

Stigma_226

Stigma_227

Stigma_228

Stigma_229
(hydrolase)

Stigma_230

Stigma_231

Stigma_232

Stigma_233

Stigma_234
(phosphoesterase)

Stigma_235

Stigma_236
(SprT-like protease)

Stigma_237

Stigma_238

Stigma_239

Stigma_240

Stigma_241

Stigma_242

Stigma_243

Stigma_244

Stigma_245

Stigma_246

Stigma_247

Stigma_248

Stigma_249

Stigma_250

Stigma_251

Stigma_252

Stigma_253

Stigma_254

Stigma_255

Stigma_256

Stigma_257

Stigma_258

Stigma_259

Stigma_260

Stigma_261

Stigma_262

Stigma_263

Stigma_264

Stigma_265

Stigma_266

Stigma_267

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON456343
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.8 x 10^9 pfu/mL
Pitt Freezer Box# 118
Pitt Freezer Box Grid# A7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload