The Actinobacteriophage Database | Phage BobtimousPrime

Mycobacterium phage BobtimousPrime

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Detailed Information for Phage BobtimousPrime
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Ryan Mandre
Year Found	2025
Location Found	Fairfax, VA United States
Finding Institution	George Mason University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	Unavailable
Discovery Notes	Very dry soil Outdoor temp 79F Slight amounts of organic material in soil Collected 9/12/25 at 12:37PM Collected 2 inches below the ground Collected on a mount of a removed tree
Naming Notes	I wanted my phage to have the power and authority it deserves, and I believe MrBOB does that.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 22, 2026
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina NextSeq 1000
Read Type	Single-end reads
Read Length	100 bp
Approximate Shotgun Coverage	736
Genome length (bp)	69151
Character of genome ends	Circularly Permuted
GC Content	57.4%
Fasta file available?	Available upon entering GPS coordinates
Characterization
Cluster	H
Subcluster	H1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Beckerton (H1) BobtimousPrime (H1) Cborch11 (H1) Damien (H1) Konstantine (H1) Megatron06 (H1) Oaker (H1) Phreeze (H1) Predator (H1) Puissant (H1) Thumb (H1) Barnyard (H2) DrLupo (H2)
Annotating Institution	George Mason University
Annotation Status	Being Annotated (Expected completion by 5/1/2026)
Plaque Notes	Created numerous very small plaques.
Morphotype	Siphoviridae
Number of Genes	93
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View BobtimousPrime_1 BobtimousPrime_2 BobtimousPrime_3 BobtimousPrime_4 BobtimousPrime_5 BobtimousPrime_6 BobtimousPrime_7 BobtimousPrime_8 BobtimousPrime_9 BobtimousPrime_10 BobtimousPrime_11 BobtimousPrime_12 BobtimousPrime_13 BobtimousPrime_14 BobtimousPrime_15 BobtimousPrime_16 BobtimousPrime_17 BobtimousPrime_18 BobtimousPrime_19 BobtimousPrime_20 BobtimousPrime_21 BobtimousPrime_22 BobtimousPrime_23 BobtimousPrime_24 BobtimousPrime_25 BobtimousPrime_26 BobtimousPrime_27 BobtimousPrime_28 BobtimousPrime_29 BobtimousPrime_30 BobtimousPrime_31 BobtimousPrime_32 BobtimousPrime_33 BobtimousPrime_34 BobtimousPrime_35 BobtimousPrime_36 BobtimousPrime_37 BobtimousPrime_38 BobtimousPrime_39 BobtimousPrime_40 BobtimousPrime_41 BobtimousPrime_42 BobtimousPrime_43 BobtimousPrime_44 BobtimousPrime_45 BobtimousPrime_46 BobtimousPrime_47 BobtimousPrime_48 BobtimousPrime_49 BobtimousPrime_50 BobtimousPrime_51 BobtimousPrime_52 BobtimousPrime_53 BobtimousPrime_54 BobtimousPrime_55 BobtimousPrime_56 BobtimousPrime_57 BobtimousPrime_58 BobtimousPrime_59 BobtimousPrime_60 BobtimousPrime_61 BobtimousPrime_62 BobtimousPrime_63 BobtimousPrime_64 BobtimousPrime_65 BobtimousPrime_66 BobtimousPrime_67 BobtimousPrime_68 BobtimousPrime_69 BobtimousPrime_70 BobtimousPrime_71 BobtimousPrime_72 BobtimousPrime_73 BobtimousPrime_74 BobtimousPrime_75 BobtimousPrime_76 BobtimousPrime_77 BobtimousPrime_78 BobtimousPrime_79 BobtimousPrime_80 BobtimousPrime_81 BobtimousPrime_82 BobtimousPrime_83 BobtimousPrime_84 BobtimousPrime_85 BobtimousPrime_86 BobtimousPrime_87 BobtimousPrime_88 BobtimousPrime_89 BobtimousPrime_90 BobtimousPrime_91 BobtimousPrime_92 BobtimousPrime_93
Publication Info
Uploaded to GenBank?	No
GenBank Accession	None yet
Refseq Number	None yet
Archiving Info
Archiving status	Not in Pitt Archives

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