| Discovery Information |
| Isolation Host | Microbacterium paraoxydans NRRL B-14843 |
| Duplicates of this Phage | Cygni |
| Found By | Nicole Grant |
| Year Found | 2021 |
| Location Found | Hudson, WI United States |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 32°C |
| GPS Coordinates | 44.984533 N, 92.7545 W Map |
| Discovery Notes | Date collected: 9/8/21 @ 7:00AM
Depth of Collection: 5 cm
Weather Conditions: 63⁰F, dry, sunny, slight wind
Sample Site Description: compost pile in alleyway, semi-moist, partially degraded leaves and grass
-No live plants nearby
-Squirrels, chipmunks, spiders, earthworms, various insects observed here
-From Karen Klyczek's soil sample |
| Naming Notes | My favorite horse from my work and volunteer barn, Main Stay Therapeutic Farm, was named Cassa. She was recently put down because of complications with her hooves, where her condition was not improving. So the best option for her was to be laid to rest to be free of any further pain and discomfort. This phage is my tribute to her, as a little version of her, with the suffix "-ita" meaning "little" in Spanish. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 26, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1220 |
| Genome length (bp) | 61868 |
| Character of genome ends | Unknown |
| Direct Terminal Repeat Length | 1649 bp |
| GC Content | 57.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | GB |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Size: about 1mm
Appearance: clear center, medium-sized turbid halo
-Relatively uniform throughout; some smaller size |
| Morphotype | Siphoviridae |
| Number of Genes | 129 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON526969 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
140 |
| Pitt Freezer Box Grid# |
B9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
