| Discovery Information |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Former names | CurlyWings |
| Found By | Tyler Nguyen, Elana Frazier |
| Year Found | 2024 |
| Location Found | Catonsville, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 39.24979 N, 76.713173 W Map |
| Discovery Notes | The soil was collected from a wooded area outside of the BWTech building on the UMBC campus on September 12th, 2024. The temperature was 22°C. The soil was dry and had a milk-chocolate-like coloration and contained ubiquitous amounts of organic debris. |
| Naming Notes | Named in honor of Kim Chae-Won, the leader of The KPop girl group LE SSERAFIM and a former member of the KPop girl group IZ*ONE. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 4, 2025 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NovaSeq X Plus |
| Read Type | Paired-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 7372 |
| Genome length (bp) | 127737 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 2010 bp |
| GC Content | 52.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BK |
| Subcluster | BK2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Chaewon is a confirmed temperate bacteriophage. Mesas were found on a plate after 7 days of incubation. These colonies were isolated and purified and were able to create clearings when patched onto wild-type S. mirabilis lawns, confirming their identity as lysogens. The lysogens are also rather resistant to superinfection, by Chaewon and a panel of other streptomyces bacteriophages. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | Being Annotated (Expected completion by 12/4/2025) |
| Plaque Notes | Clear plaques ranging from 1.3 mm to 2 mm after 24 hours, develops a light-colored border after 48 hours, all with S. mirabilis |
| Morphotype | Siphoviridae |
| Number of Genes | 223 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
211 |
| Pitt Freezer Box Grid# |
H8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
