| Detailed Information for Phage Chop | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Jackson Lipscomb |
| Year Found | 2021 |
| Location Found | Bismark, AR United States |
| Finding Institution | Ouachita Baptist University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.309953 N, 93.151496 W Map |
| Discovery Notes | This phage was found in a soil sample collected from a home garden. |
| Naming Notes | As the academic year progressed, "Chop" became the term for an alpha-hunter-type phage. What started as an idea of a warrior/defensive linemen soon became a way to motivate us to find the Holy Grail of phages. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 19, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2221 |
| Genome length (bp) | 50919 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TGCCGCGGTA |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DI |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Ouachita Baptist University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques formed are usually 1.5-2mm in diameter. Comet-like tails are also noticeable in bigger plaques. This phage also shows an ability to make <1mm plaques, which was enhanced when incubated at 37 degrees Celsius. |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewChop_1 Chop_2 Chop_3 Chop_4 Chop_5 Chop_6 Chop_7 Chop_8 Chop_9 Chop_10 Chop_11 Chop_12 Chop_13 Chop_14 Chop_15 Chop_16 Chop_17 Chop_18 Chop_19 Chop_20 Chop_21 Chop_22 Chop_23 Chop_24 Chop_25 Chop_26 Chop_27 Chop_28 Chop_29 Chop_30 Chop_31 Chop_32 Chop_33 Chop_34 Chop_35 Chop_36 Chop_37 Chop_38 Chop_39 Chop_40 Chop_41 Chop_42 Chop_43 Chop_44 Chop_45 Chop_46 Chop_47 Chop_48 Chop_49 Chop_50 Chop_51 Chop_52 Chop_53 Chop_54 Chop_55 Chop_56 Chop_57 Chop_58 Chop_59 Chop_60 Chop_61 Chop_62 Chop_63 Chop_64 Chop_65 Chop_66 Chop_67 Chop_68 Chop_69 Chop_70 Chop_71 Chop_72 Chop_73 Chop_74 Chop_75 Chop_76 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON637763 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 8.8 * 10^8 pfu/mL |
| Pitt Freezer Box# | 135 |
| Pitt Freezer Box Grid# | E11 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |