Gordonia phage Parada
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Detailed Information for Phage Parada
Discovery Information
Isolation HostGordonia terrae 3612
Found ByPraise Olatunde
Year Found2016
Location FoundGrand Rapids, MI USA
Finding InstitutionCalvin College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.933611 N, 85.590833 W Map
Discovery Notes-Soil sample obtained from under the squash plants in the Calvin Campus Garden in Sept.​
-Moist, wet dark brown dirt
Naming NotesName comes from the Yoruba Language
Meaning in English is : "renewing"
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 4, 2017
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage539
Genome length (bp)49909
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTGCCGCGGTA
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDI
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusIn GenBank
Plaque Notes-Plaques on my spot test were about 9.5mm in diameter​
-Plaques were symmetrical small circles​
-Clear (suggests lytic)​
-Consistent pattern in the different dilutions of the purification procedure​
-When my g.terrae lawn were richer the plaques were slightly larger​
-plaques are about 2 mm in size
Has been Phamerated?Yes
Gene List

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Parada_1

Parada_2

Parada_3

Parada_4

Parada_5
(terminase small subunit)

Parada_6
(terminase large subunit)

Parada_7
(portal protein)

Parada_8
(capsid maturation protease)

Parada_9
(scaffolding protein)

Parada_10
(major capsid protein)

Parada_11

Parada_12
(head-to-tail connector complex protein)

Parada_13
(head-to-tail stopper)

Parada_14
(tail terminator)

Parada_15

Parada_16
(major tail protein)

Parada_17

Parada_18
(tail assembly chaperone)

Parada_19
(tail assembly chaperone)

Parada_20
(tape measure protein)

Parada_21
(minor tail protein)

Parada_22
(minor tail protein)

Parada_23
(minor tail protein)

Parada_24
(minor tail protein)

Parada_25
(minor tail protein)

Parada_26

Parada_27

Parada_28

Parada_29
(lysin A, protease C39 domain)

Parada_30
(lysin A, glycosyl hydrolase domain)

Parada_31
(membrane protein)

Parada_32
(membrane protein)

Parada_33

Parada_34
(HicB-like antitoxin)

Parada_35
(HicA-like toxin)

Parada_36

Parada_37

Parada_38

Parada_39
(integrase)

Parada_40
(immunity repressor)

Parada_41
(helix-turn-helix DNA binding domain protein)

Parada_42
(helix-turn-helix DNA binding domain protein)

Parada_43

Parada_44

Parada_45

Parada_46

Parada_47
(exonuclease)

Parada_48

Parada_49

Parada_50

Parada_51

Parada_52

Parada_53

Parada_54

Parada_55

Parada_56

Parada_57

Parada_58

Parada_59

Parada_60

Parada_61

Parada_62

Parada_63
(metallophosphoesterase)

Parada_64

Parada_65
(hydrolase)

Parada_66
(DNA methylase)

Parada_67

Parada_68

Parada_69
(DNA primase)

Parada_70
(endonuclease VII)

Parada_71

Parada_72
(helix-turn-helix DNA binding domain protein)

Parada_73
(HNH endonuclease)

Parada_74

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359319
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.0 x 10^10
Date of SEA Lysate Titering Nov 4, 2016
Pitt Freezer Box# 39
Pitt Freezer Box Grid# A11
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload