| Detailed Information for Phage Circinus |
| Discovery Information |
| Isolation Host | Streptomyces griseofuscus ATCC 23916 |
| Found By | Amy Kwan, Caelan Miller, & Brandon Tang |
| Year Found | 2017 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 38.6032 N, 90.2616 W Map |
| Discovery Notes | Specimen was discovered near a tree by the Seed Sprout Spoon, located in St. Louis, MO. |
| Naming Notes | The name is inspired by the constellation Circinus and the plaque's stellar shape. Circinus means "the compass" from its translation from Latin. All of our previous samples proved to be difficult in cultivating phage growth until this sample gave us a sense of direction through its successful plaque growth. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 12, 2018 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 86 |
| Genome length (bp) | 126383 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 2056 bp |
| GC Content | 52.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BK |
| Subcluster | BK2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Stellar in shape, clear around edges and within plaque, preferentially medium-sized |
| Morphotype | Siphoviridae |
| Number of Genes | 216 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK620896 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.0*10^9 |
| Pitt Freezer Box# |
49 |
| Pitt Freezer Box Grid# |
C5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
