| Detailed Information for Phage Erk16 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emily R Konrad |
| Year Found | 2016 |
| Location Found | Oxford, OH USA |
| Finding Institution | Miami University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.502689 N, 84.732361 W Map |
| Discovery Notes | Oxford, OH Date sample was taken: 9-7-16 clay-like soil containing some organic matter (small pebbles, small pieces of mulch) taken from approx. 3 cm below surface |
| Naming Notes | initials, hs grad class |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 28, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 223 |
| Genome length (bp) | 64877 |
| Character of genome ends | Circularly Permuted |
| GC Content | 59.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | D |
| Subcluster | D1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | lytic |
| Other Cluster Members |
Click to ViewAdjutor (D1) BigMama (D1) Butterscotch (D1) Chill (D1) Delton (D1) Erk16 (D1) Giuseppe (D1) Gumball (D1) Helpful (D1) KandZ (D1) Mopey (D1) Nova (D1) PBI1 (D1) Penelope2018 (D1) Phalaborwa (D1) PLot (D1) Prager (D1) SirHarley (D1) SuperheroCarly (D1) Thoth (D1) Troll4 (D1) Visconti (D1) WaldoWhy (D1) Hawkeye (D2) |
| Annotating Institution | Miami University |
| Annotation Status | In GenBank |
| Plaque Notes | approx. 2 mm plaque diameter; during initial rounds of purification, plaques had turbid halos; morphology changed from "starburst" margin to nearly perfectly circular |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewErk16_1 Erk16_2 Erk16_3 Erk16_4 Erk16_5 Erk16_6 Erk16_7 Erk16_8 Erk16_9 Erk16_10 Erk16_11 Erk16_12 Erk16_13 Erk16_14 Erk16_15 Erk16_16 Erk16_17 Erk16_18 Erk16_19 Erk16_20 Erk16_21 Erk16_22 Erk16_23 Erk16_24 Erk16_25 Erk16_26 Erk16_27 Erk16_28 Erk16_29 Erk16_30 Erk16_31 Erk16_32 Erk16_33 Erk16_34 Erk16_35 Erk16_36 Erk16_37 Erk16_38 Erk16_39 Erk16_40 Erk16_41 Erk16_42 Erk16_43 Erk16_44 Erk16_45 Erk16_46 Erk16_47 Erk16_48 Erk16_49 Erk16_50 Erk16_51 Erk16_52 Erk16_53 Erk16_54 Erk16_55 Erk16_56 Erk16_57 Erk16_58 Erk16_59 Erk16_60 Erk16_61 Erk16_62 Erk16_63 Erk16_64 Erk16_65 Erk16_66 Erk16_67 Erk16_68 Erk16_69 Erk16_70 Erk16_71 Erk16_72 Erk16_73 Erk16_74 Erk16_75 Erk16_76 Erk16_77 Erk16_78 Erk16_79 Erk16_80 Erk16_81 Erk16_82 Erk16_83 Erk16_84 Erk16_85 Erk16_86 Erk16_87 Erk16_88 Erk16_89 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH316562 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.93 X 10^10 |
| Pitt Freezer Box# | 31 |
| Pitt Freezer Box Grid# | F8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
